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Control products, reagents, and RNA samples for use in the study of RNA and the process of RNA interference (RNAi or post-transcriptional gene silencing).
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Firefly Luciferase mRNA (ARCA 5mCTP UTP) encodes firefly luciferase protein originally isolated from Photinus pyralis Firefly luciferase is a widely used bioluminescence reporter molecule for studies on gene regulation cell viability ATP levels and in vivo imaging In the presence of ATP and oxygen luciferase catalyzes the oxidation of D-Luciferin (Cat C3654) emitting chemiluminescence at 560 nm This product is a 5 -capped 3 -poly(A)-tailed mRNA modified with 5mCTP and UTP mimicking mature eukaryotic mRNA Capped with ARCA (anti-reverse cap analog Cat B8175) via co-transcription it forms a Cap 0 structure enhancing stability and translation efficiency [1 2] The 5mCTP (Cat B7967) and UTP (Pseudo-UTP Cat B7972) modifications reduce innate immune activation and increase mRNA stability The poly(A) tail boosts stability and translation initiation efficiency
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The luciferase-luciferin bioluminescent system provides a highly sensitive and efficient tool for detecting gene expression In the presence of ATP oxygen and Mg2 firefly luciferase catalyzes the oxidation reaction of luciferin into oxyluciferin accompanied by the emission of bioluminescence at about 560 nm following detection by a luminometer or liquid scintillation counter The Single Firefly Luciferase Reporter Gene Assay Kit allows direct quantification of intracellular firefly luciferase activity via chemiluminescence without the need for washing or harvesting cells This assay is a one-step detection kit providing exceptional sensitivity high signal stability and ease of use This product is an alternative lyophilized powder version of Kit (K2236) with the same performance offering the benefit of long-term stable storage at -20 C Before use the lyophilized substrate powder needs to be fully dissolved with the buffer provided in the kit to prepare working solution
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Negative control pool of four siRNAs designed and microarray tested for minimal targeting of human, mouse or rat genes. ON-TARGETplus modifications reduce potential off-targets. Recommended for determination of baseline cellular responses in RNAi experiments
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Glucose-6-Phosphate Dehydrogenase Primary Antibody Unconjugated Public Immunogen Range 460-515/515 Host Species Rabbit Target Species Human Clonality Monoclonal Applications WB FCM IC Concentration Lot dependent Size 50 ul Storage Buffer Supplied in PBS (pH 7 4) containing 50% glycerol and 0 02% sodium azide
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Dyneins are a group of microtubule-activated ATPases that function as molecular motors They are divided into two subgroups of axonemal and cytoplasmic dyneins The cytoplasmic dyneins function in intracellular motility including retrograde axonal transport protein sorting organelle movement and spindle dynamics Molecules of conventional cytoplasmic dynein are comprised of 2 heavy chain polypeptides and a number of intermediate and light chains This gene encodes a member of the cytoplasmic dynein heavy chain family
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This gene encodes a protein which contains a conserved nuclear localization signal. The encoded protein may have a role in spermatogenesis, cell cycle control, and in meiotic cell division. [provided by RefSeq, Jul 2008].
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