Protein Analysis Reagents
Thermo Scientific™ Trypsin, TPCK Treated
Thermo Scientific Pierce TPCK Trypsin is a serine endoprotease that is applicable to amino acid analysis and protein sequencing, mapping and structural studies; the immobilized form allows sample separation after treatment.
MP Biomedicals™ Pepsin
Pepsin is a peptidase used to digest proteins and is commonly used in the preparation of Fab fragments from antibodies. Pepsin, from porcine gastric mucosa, has been used to hydrolyze dry cervical samples in mice
Thermo Scientific™ EZ-Link™ Maleimide Activated Horseradish Peroxidase
Activated horseradish peroxidase (HRP) enzyme and kit to label proteins, peptides or other ligands via sulfhydryl groups (reduced cysteines) to make HRP conjugates.
Thermo Scientific™ NHS (N-hydroxysuccinimide)
Prepare amine-reactive NHS-ester crosslinkers or enhance carbodiimide (EDC) conjugation for peptides or proteins using N-hydroxysuccinimide, CAS 6066-82-6.
BD Perm/Wash™ Buffer
Can be used in intracellular cytokine staining to permeabilize cells and as an antibody diluent and cell wash buffer
Thermo Scientific™ Sodium Dodecyl Sulfate (SDS), Lauryl
Perform routine protein electrophoresis and cell lysis methods using this popular, standard-grade SDS detergent.
Thermo Scientific™ Zeba™ Dye and Biotin Removal Spin Columns and Filter Plates, 0.5–10 mL
Enable fast and efficient removal of non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers from protein samples with Thermo Scientific Pierce Zeba Dye and Biotin Removal columns and filter plates.
Thermo Scientific™ Pierce™ Traut's Reagent (2-iminothiolane)
2-Iminothiolane-HCl (CAS 4781-83-3); an amine-reactive thiolation reagent to cap protein primary amines with sulfhydryl groups for immediate crosslinking.
Thermo Scientific™ Guanidine-HCl
Pure, crystalline guanidium hydrochloride (GdnHCl, GuHCl), CAS 50-01-1; denaturant for protein biology methods (elution, solubilization, refolding, storage).
Thermo Scientific™ Brij™-35, 30% Solution
Stabilized Brij-35 detergent provided as 10% and 30% solutions for use in various protein methods including cell lysis and HPLC.
Thermo Scientific™ Pierce™ Bovine Gamma Globulin Standard Ampules, 2 mg/mL
High-quality, calibrated BGG solutions (2mg/mL) to serially dilute to create protein assay standard curves to measure antibody (IgG) concentration.
BD Hoechst 33342 Solution
Bioimaging certified reagent for fluorescent staining of DNA and nuclei in live or fixed cells
Thermo Scientific™ Urea
Pure, crystalline urea (CH4N2O), CAS 57-13-6; denaturant with low UV-absorptivity for protein biology methods (elution, solubilization, refolding, storage).
Invitrogen™ One Shot™ BL21 Star™ (DE3) Chemically Competent E. coli
One Shot BL21 Star (DE3) Chemically Competent E. coli are designed for applications that require high-level expression of non-toxic recombinant proteins from low copy number, T7-promoter-based expression systems such as Champion pET vectors.
Thermo Scientific™ Pierce™ Horseradish Peroxidase
Purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and immunohistochemistry applications.