Restriction Enzymes
Supply Solutions Millipore Sigma - Roche Transcriptor Reverse Transcriptase, 1 PK
Millipore Sigma - Roche Transcriptor Reverse Transcriptase, 1 PK
New England Biolabs, Inc. BceAI – 50 units
A restriction endonuclease that recognizes the sequence ACGGCNNNNNNNNNNNN^NN_.
New England Biolabs, Inc. PciI – 1000 units
A restriction endonuclease that recognizes the sequence A^CATG_T.
New England Biolabs, Inc. I-SceI – 500 units
A restriction endonuclease that recognizes the sequence TAGGG_ATAA^CAGGGTAAT. I-SceI is an intron-encoded endonuclease. The intron encoding I-SceI is present in the mitochondria of Saccharomyces cerevisiae.
New England Biolabs, Inc. XbaI – 15000 units
A restriction endonuclease that recognizes the sequence T^CTAG_A.
New England Biolabs, Inc. BssSI-v2 – 200 units
BssSI-v2 is an engineered and improved version of BssSI that recognizes the sequence VC^TCGAGB. BssSI-v2 is an engineered and improved version of BssSI. (NEB uses the designation "v2" or "HF" in the name to indicate engineered enzymes.)
New England Biolabs, Inc. BglII – 2000 units
A restriction endonuclease that recognizes the sequence A^GATC_T.
New England Biolabs, Inc. BstBI – 2500 units
A restriction endonuclease that recognizes the sequence TT^CG_AA.
New England Biolabs, Inc. APE 1 – 5000 units
Human apurinic/apyrimidinic (AP) endonuclease, APE 1, also known as HAP 1 or Ref-1, shares homology with Escherichia coli exonuclease III protein. APE 1 cleaves the phosphodiester backbone immediately 5 to an AP site, via hydrolytic mechanism, to generate a single-strand DNA break leaving a 3-hydroxyl and 5-deoxyribose phosphate terminus. Besides AP endonuclease activity, APE 1 has also been reported to have weak DNA 3 -diesterase, 3 to 5 exonuclease and RNase H activities (3-5). In addition to DNA repair activity, APE 1 is also capable of regulating the DNA binding activity of many transcription factors in vitro by a redox mechanism. As part of this process, APE 1 has been shown to stimulate the DNA binding activity of Fos-Jun heterodimers, Jun-Jun homodimers and Hela cell AP-1 proteins as well as that of several other transcription factors including NF-kB, Myb and members of the ATF/CREB family.
New England Biolabs, Inc. HaeII – 2000 units
A restriction endonuclease that recognizes the sequence R_GCGC^Y.
New England Biolabs, Inc. AseI – 2000 units
A restriction endonuclease that recognizes the sequence AT^TA_AT.
New England Biolabs, Inc. AatII – 2500 units
A restriction endonuclease that recognizes the sequence G_ACGT^C.
New England Biolabs, Inc. NotI-HF® – 2500 units
A high fidelity restriction endonuclease that recognizes the sequence GC^GGCC_GC. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
- Reduced star activity
- Supplied with 1 vial of Gel Loading Dye, Purple (6X)
New England Biolabs, Inc. SwaI – 2000 units
A restriction endonuclease that recognizes the sequence ATTT_^AAAT.