Restriction Enzymes
New England Biolabs, Inc. PshAI – 5000 units
A restriction endonuclease that recognizes the sequence GACNN_^NNGTC.
New England Biolabs, Inc. AclI – 300 units
A restriction endonuclease that recognizes the sequence AA^CG_TT.
New England Biolabs, Inc. TspRI – 1000 units
A restriction endonuclease that recognizes the sequence _NNCASTGNN^.
New England Biolabs, Inc. BsoBI – 10000 units
A restriction endonuclease that recognizes the sequence C^YCGR_G.
New England Biolabs, Inc. BciVI – 1000 units
A restriction endonuclease that recognizes the sequence GTATCCNNNNN_N^.
New England Biolabs, Inc. BbvCI – 500 units
A restriction endonuclease that recognizes the sequence CC^TCA_GC.
New England Biolabs, Inc. PflFI – 2000 units
A restriction endonuclease that recognizes the sequence GACN^N_NGTC.
New England Biolabs, Inc. NspI – 250 units
A restriction endonuclease that recognizes the sequence R_CATG^Y.
New England Biolabs, Inc. NsiI-HF – 5000 units
A high fidelity restriction endonuclease that recognizes the sequence ATGCA^T. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. BstEII-HF – 2000 units
A high fidelity restriction endonuclease that recognizes the sequence G^GTNACC. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
New England Biolabs, Inc. MspJI – 1000 units
A restriction endonuclease that recognizes the sequence CNNRNNNNNNNNN^NNNN_.
New England Biolabs, Inc. BtsIMutI – 100 units
A restriction endonuclease that recognizes the sequence CAGTG_NN^.
New England Biolabs, Inc. PvuII-HF – 25000 units
A high fidelity restriction endonuclease that recognizes the sequence CAG^CTG. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. ScaI-HF – 5000 units
A high fidelity restriction endonuclease that recognizes the sequence AGT^ACT. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.