Singleplex and Multiplex Bead Based Immunoassays
Meso Scale Discovery V-PLEX Plus Human MDC Kit
The V-PLEX Plus Human MDC Kit from Meso Scale Discovery is a multiplex immunoassay kit that allows for the simultaneous detection and quantification of 10 human cytokines and chemokines (IL-2 IL-4 IL-5 IL-10 IL-12p70 IL-13 IFN- TNF- IP-10 and MCP-1) from cell culture supernatants serum plasma and other biological samples The kit utilizes MSD s electrochemiluminescent detection technology and provides high sensitivity a wide dynamic range and requires low sample volumes The kit includes a 96-well plate coated with capture antibodies detection antibodies read buffer wash buffer diluent calibrators and controls
Meso Scale Discovery UPLEX HUM ICAM1 ASY384well
The Meso Scale Discovery U-PLEX Human ICAM-1 384-Well Assay Kit is a multiplex immunoassay that measures Intercellular Adhesion Molecule 1 (ICAM-1) in human serum plasma and cell culture supernatants This kit uses electrochemiluminescence detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample consumption It is available in a 384-well plate format includes reagents for up to 80 tests and has an assay time of 3 5 hours This assay kit can be used for biomarker research and drug discovery applications related to inflammation immune response and endothelial cell biology
Meso Scale Discovery S-PLEX Human TNF-B KitQP
The Meso Scale Discovery S-PLEX Human KinekineTM Panel 1 (22-plex) is a multiplex immunoassay that simultaneously measures 22 human cytokines chemokines growth factors and immune modulators in serum plasma and culture supernatants This assay uses electrochemiluminescence detection technology on Meso Scale Discovery s proprietary multi-array plate platform The panel includes IL-1 IL-2 IL-4 IL-5 IL-6 IL-7 IL-8 IL-10 IL-12p70 IL-13 IL-15 IL-16 IL-17A IFN- TNF- TNF- GM-CSF VEGF-A CCL2 CCL3 CCL4 and CCL11 This assay allows high-throughput sensitive and reproducible quantification of multiple analytes from a small sample volume
Meso Scale Discovery U-PLEX NHP IL-1RA ASY384-well
The Meso Scale Discovery U-PLEX NHP IL-1ra 384-Well Assay Kit measures human interleukin-1 receptor antagonist (IL-1ra) in serum plasma and cell culture supernatants using electrochemiluminescence (ECL) detection It offers high sensitivity broad dynamic range and precision A Meso Scale Discovery SECTOR Imager is required The kit includes a 384-well plate with IL-1ra capture antibody detection antibody buffers and calibrators Sensitivity is 0 08 pg/mL with an upper quantification limit of 2500 pg/mL
Meso Scale Discovery UPLEX HUM GIP active ASY384
The Meso Scale Discovery U-PLEX Human GIP Active 384-Well Assay is a 384-well electrochemiluminescent immunoassay for the quantitative measurement of active glucagon-like peptide-1 (GIP) in human serum plasma and cell culture supernatants It utilizes MSD s proprietary SULFO-TAG labels and multi-array technology to provide sensitive and reproducible results in just 3 5 hours The assay has a lower limit of detection of 0 07 pg/mL and an upper limit of quantification of 2500 pg/mL It requires just 25 L of sample per well and includes reagents for a 384-well plate
Innovative Research Inc Hmn PAI1 and tPA Cmplx Kit
Human PAI-1 and tPA Complex ELISA Kit - This kit is for the quantitative determination of the covalent complex of tPA and its inhibitor PAI-1 in human plasma, serum, urine, cell culture media, or tissue extracts. For RUO. Strip well format. Reagents for up to 96 tests. Human PAI-1 in samples will bind to the monoclonal capture antibody coated on the microtiter plate. Free, latent, and complexed PAI-1 will react with the capture antibody. After washing, polyclonal anti-human tPA primary antibody binds to the captured PAI-1/tPA complex. Excess primary antibody is washed away and bound antibody is then reacted with peroxidase conjugated secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of PAI-1/tPA complex. Color is proportional to the concentration of PAI-1/tPA complex. Free tPA and PAI-1 will not be detected by this assay.
Meso Scale Discovery U-PLEX Rat C-Peptide ASYQP
The Meso Scale Discovery U-PLEX Rat C-Peptide Assay Kit is a multiplex immunoassay for measuring C-peptide in rat serum plasma and other samples It uses MSD s electrochemiluminescent detection for high sensitivity and a broad dynamic range with low sample volume The kit includes a 96-well plate with capture antibodies detection antibodies with MSD SULFO-TAG calibrators and diluent Sensitivity is 1 3 pg/mL with a quantification limit of 10 000 pg/mL Results are available in 3 5 hours using an MSD instrument like the MESO QuickPlex SQ 120
Iwai North America ALyS505NK-EX: Xeno-free (Serum free) cell culture mediums for expansion of Human NK cells, without IL-2. Size: 1L
ALyS505NK-EX is xeno-free cell culture medium without IL-2, optimized for human natural killer (NK) cells derived from peripheral blood mononuclear cells (PBMCs). ALyS NK-EX Acts as a ‘copy machine’ for the highly active NK cells. In many cases, in vitro cultivation of NK cells, often results in a low expansion rate, an exhausted phenotype due to long-term expansion, and overgrowth of T cells if present in the initial culture. By employing ALyS505NK-AC for activation and ALyS505NK-EX for expansion, one can attain a highly pure, NK-rich population of cells in 14 days without the need for sorting with beads or flow cytometry.1. Niu, C. et al. Low-dose bortezomib increases the expression of NKG2D and DNAM-1 ligands and enhances induced NK and γδ T cell-mediated lysis in multiple myeloma. Oncotarget 8, 5954–5964 (2017) doi: 10.18632/oncotarget.13979.
Meso Scale Discovery S-PLEX NHP CTLA-4 Kit 25 PL
The Meso Scale Discovery CTLA-4 Discovery Kit is a 96-well plate assay that measures the levels of CTLA-4 in biological samples such as serum plasma cell culture supernatants cell lysates or other biological fluids It uses electrochemiluminescence (ECL) technology and provides high sensitivity broad dynamic range and excellent reproducibility The assay takes approximately 3 5 hours to complete and requires a Meso Scale Discovery SECTOR Imager for readout This product allows for the quantitative measurement of CTLA-4 which plays an important role in downregulating immune responses
Meso Scale Discovery V-PLEX NHP IL-7 Kit 25Plate
The Meso Scale Discovery V-PLEX NHP IL-7 Kit is an electrochemiluminescent immunoassay for the quantitative measurement of human interleukin 7 (IL-7) in serum plasma and cell culture supernatants The assay uses MSD s proprietary SULFO-TAG labels and multi-array technology to provide sensitive and reproducible results with a detection range of 0 06-1000 pg/mL and a lower limit of detection of 0 03 pg/mL The kit includes a 96-well plate coated with capture antibody detection antibody conjugated to ruthenium calibrators diluent wash buffer read buffer and plate seals The assay takes approximately 3 5 hours to complete
Meso Scale Discovery UPLEX MSE IFN ASY384well
The Meso Scale Discovery U-PLEX Mouse IFN gamma 384-well Assay Kit is a 384-well electrochemiluminescent immunoassay for the quantitative measurement of mouse interferon gamma (IFN) in cell culture supernatants serum plasma and other biological matrices The assay utilizes MSD s proprietary SULFO-TAG labels and multi-array plate technology to provide high sensitivity broad dynamic range and minimal matrix interference The kit includes a 384-well plate coated with capture antibody detection antibody conjugated to ruthenium label calibrators and buffer The assay has a typical sensitivity of 0 3 pg/mL and an upper limit of quantification of 10 000 pg/mL
Meso Scale Discovery UPLEX HUM RANKL/TNFSF11 ASY
The Meso Scale Discovery U-PLEX Human RANKL (TNFSF11) Assay Kit is a multiplex immunoassay for measuring RANKL in human serum plasma and cell culture supernatants It uses electrochemiluminescence (ECL) detection for high sensitivity broad dynamic range and precision The kit includes a 96-well plate with capture antibodies detection antibodies with MSD SULFO-TAG calibrators and controls Sensitivity is 0 04 pg/mL with an upper quantification limit of 10 000 pg/mL enabling accurate RANKL quantitation for research like biomarker discovery and validation
Meso Scale Discovery UPLEX HUM MIP1 ASY384well
The Meso Scale Discovery U-PLEX Human MIP-1alpha 384-Well Assay Kit is a multiplex immunoassay for measuring human MIP-1alpha in serum plasma and cell culture supernatants It uses electrochemiluminescence detection offering high sensitivity a wide dynamic range and low sample volume needs The kit includes a 384-well plate with capture antibodies detection antibodies with electrochemiluminescent labels calibrators and optimized diluents It enables simultaneous measurement of MIP-1alpha and other biomarkers ensuring high performance and reliability in biomarker quantification
Meso Scale Discovery V-PLEX GIP active Kit
The Meso Scale Discovery V-PLEX GIP Active Kit is a human GIP immunoassay kit that enables the quantitative measurement of active GIP in cell culture supernatants serum plasma and other biological samples The electrochemiluminescent assay utilizes MSD s proprietary SULFO-TAG labels and multi-array plate technology to provide high sensitivity wide dynamic range and rapid results The kit includes a 96-well plate coated with a GIP capture antibody detection antibody conjugated to ruthenium calibrators and reagents required to complete the assay The active GIP kit allows sensitive reproducible quantification of active GIP levels with a lower limit of detection of 0 07 pg/mL