Advanced ImmunoChemical, Inc.

Advanced ImmunoChemical, Inc. Influenza A (H1N1) Virus (Strain A/Beijing/262/95), 0.1mg

This item is manufactured by HyTest, Ltd and distributed by Advanced ImmunoChemical, Inc. Antigens/ Infectious Diseases/ Influenza A, (H1N1) virus (strain A/Beijing/262/95). Source:Allantoic fluid of 10 days old embryonated eggs, inoculated with influenza A virus, strain A/Beijing/262/95.Applications:Serological studies of influenza A virus, immunogen for antibody production.Analysis:Purity > 90 %Immunological activity:Tested with anti-influenza A monoclonal antibodies ( Cat. # 5-IVAN) in ELISA.Purification:Ultracentrifugation with 10 – 40 % sucrose gradient.Presentation:Presentation: STE, 0.09 % sodium azide (NaN3) and 0.005 % thimerosal.Inactivation:Thimerosal and beta propiolactone treatment. This product has been treated in a manner consistent with methods of inactivation. Generally accepted good laboratory practices appropriate to microbiological/viral safe handling practices and techniques are required at work.Storage Conditions:-20 °C (between -15 and -30 °C allowed).

Advanced ImmunoChemical, Inc. CA72-4, Source - Huma Metastatic Liver Carcinoma, 250 kIU

This item is manufactured by HyTest, Ltd and distributed by Advanced ImmunoChemical, Inc. Antigens / Tumor Markers / CA72-4. Source: Human metastatic liver carcinoma.Blood sample from the tissue donor was tested and found negative for HBsAg, HIV-1 and HIV-2 antibodies and HCV.Storage: -20 °C (-15 … -30 °C allowed). Applications: Immunogen, calibrator in immunoassays, in vitro studies.Presentation: PBS, pH 7.4, 0.05 % of sodium azide (NaN₃).Material safety note: This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material. Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Advanced ImmunoChemical, Inc. Human Papillomavirus L1 Protein (HPVL1), Recombinant, Type 16, Source:Purity > 90 %, 0.1mg

This item is manufactured by HyTest, Ltd and distributed by Advanced ImmunoChemical, Inc. Antigens / Infectious Diseases / Human Papillomavirus L1 protein (HPVL1), recombinant, type 16. Source: Purity > 90 % (SDS-PAGE).Each lot is tested for protein content (Lowry method), lipid content (Total lipid), purity (SDS-PAGE), carbohydrate content (Anthrone method), antigen content (Immunoblot). Immunological activity: Tested in quantitative immunoblot with anti-HPVL1 antibodies. Purification: Purification is conducted from broken yeast cells by following scheme: clarification, ammonium sulfate precipitation, ultrafiltration, cation exchange chromatography, zonal centrifugation, size- exclusion chromatography, sterile filtration. Presentation: 20 mM KH2PO4 (pH 7.2), 320 mM NaCl, 1.7 mM EDTA, 0.01% Tween-80, 10 mM histidine. Storage Conditions: + 4 °C (between +2 and +8 °C allowed)

Advanced ImmunoChemical, Inc. ANTI-IGFBP-4 MAB

MONOCLONAL ANTIBODIES/MAbs IBP144, IBP154 and IBP180:human IGFBP-4; human N-terminal IGFBP-4 fragment (NT-IGFBP-4)MAbs IBP182, IBP185 and IBP190:human IGFBP-4; human C-terminal IGFBP-4 fragment (CT-IGFBP-4)MAb IBP3:human NT-IGFBP-4, cross-reaction with full-length human IGFBP-4MAb IBP163:human CT-IGFBP-4, cross-reaction with full-length human IGFBP-4 <5% (ELISA)Detection of human IGFBP-4, NT-IGFBP-4, and CT-IGFBP-4. MAbs are working in direct ELISA and sandwich immunoassay.Recommended pairs for human IGFBP-4 sandwich immunoassay (capture-detection): IBP185 –IBP154 IBP182 – IBP144 IBP144 – IBP190 Recommended pairs for specific human NT-IGFBP-4 sandwich immunoassay (capture-detection):IBP3 – IBP144 IBP3 – IBP180 Recommended pairs for specific human CT-IGFBP-4 sandwich immunoassay (capture-detection): IBP182 – IBP163Purification:Chromatography on protein A SepharosePresentation:PBS, pH 7.4, 0.1 % sodium azide (NaN3)Storage Conditions:+ 4 °C

Advanced ImmunoChemical, Inc. ANTI-RAT PROINSULIN MAB

MONOCLONAL ANTIBODIES/Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with fragment of rat proinsulin conjugated with a carrier protein.Specificity:Rat proinsulinMAb Isotypes:IgG1 for MAb CCI-17Applications:MAb recognizes rat proinsulin in ELISA and Sandwich type immunoassay. MAb binds specifically to proinsulin and does not bind to insulin or C-peptide. See our “Rat C-peptide and proinsulin” Assay Notes for more information. For sandwich immunoassay it is recommended to use MAb in pair with MAbs specific to N-terminal part of C-peptides I and II (see also the data sheet for Cat.# 1-CP-r and “Rat C-peptide and proinsulin” Assay Notes for more information).The recommended pair for sandwich-type rat proinsulin I and II immunoassay is (capture- detection): CCI-17 – CII-55 Purification:Chromatography on protein A SepharosePresentation:Presentation: PBS, pH 7.4, 0.1 % sodium azide (NaN3)

Advanced ImmunoChemical, Inc. ANTI-IMMUNE COMPLEX MAB

MONOCLONAL ANTIBODIES/MAbs:Ab-BNP2ccHybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with immune complex formed by Fab-fragment of BNP-specific MAb 24C5 (Cat.# 2-BNP) and synthetic human BNP.Specificity:Human BNP and proBNP in immune complex with MAb 24C5MAb Isotypes:IgG2a for MAb Ab-BNP2ccApplications:BNP and proBNP sandwich immunoassays (detection), only as a pair to MAb 24C5.Purification:Protein A affinity chromatographyPresentation:PBS, pH 7.4, 0.09 % sodium azide (NaN3)Storage Conditions:+ 4 °C

Advanced ImmunoChemical, Inc. ANTI-KIDNEY INJURY MOLECULE

MONOCLONAL ANTIBODIES/MAbs:KIM70, KIM75Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with human recombinant KIM-1 ectodomain expressed in baculovirus system.Specificity:Human KIM-1MAb Isotypes:IgG1 for MAbs KIM70, KIM75Applications:MAbs KIM70, KIM75 react with human recombinant KIM-1 in sandwich ELISA and Western blotting.Recommended pairs KIM-1 immunodetection in sandwich immunoassay (capture-detection):KIM70 – KIM75Purification:Chromatography on protein A SepharosePresentation:PBS, pH 7.4, 0.1 % sodium azide (NaN3)Storage Conditions:+ 4 °C

Advanced ImmunoChemical, Inc. ANTI-CA-19-9 MAB

MONOCLONAL ANTIBODIES/Monoclonal antibodies / Tumor Markers / Monoclonal mouse anti-CA19-9. MAbs:X91 Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with affinity purified human CA19-9 antigen. Specificity: CA19-9 antigen. MAb Isotypes: IgM for MAb X91Applications: Detection of CA19-9 antigen in immunoassays. MAbs are working in immunohistochemistry. Purification: Ion exchange chromatography. Presentation: 0.15 M NaCl, 10 mM Tris, pH 7.5, 0.05% sodium azide (NaN3). Storage Conditions:+ 4 °C.

Advanced ImmunoChemical, Inc. ANTI-GAPDH MAB

MONOCLONAL ANTIBODIES/MAbs in vitro:6C5ccMAbs in vivo:4G5Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with rabbit muscle GAPDH.Specificity:Cross-reaction of MAbs in Western blotting with GAPDH from different animal species. MAb isotypes:IgG1 for all MAb 6C5cc, 4G5Applications:GAPDH immunoassay, Western blotting, and immunocytochemistry. Purification:Protein A chromatographyPresentation:PBS, pH 7.4, 0.09% sodium azide (NaN3) Storage Conditions:+4 °C (+2 …. +8 Celsius allowed)

Advanced ImmunoChemical, Inc. NT-PROBNP, HUMAN RECOMBINANT

ANTIGENS/Source:Recombinant E.coli.Applications:Calibrator or standard in immunoassays, immunogen for antibody production.Analysis:Purity > 95 % (Tricine-SDS-PAGE).Concentration is determined spectrophotometrically.Immunoreactivity is confirmed by reaction with monoclonal antibodies specific to different fragments of NT-proBNP molecule.Predicted molecular mass 8589 Da confirmed by MALDI-MS.Recombinant human NT-proBNP contains additional methionine residue at the N-terminus (in comparison with native NT-proBNP).Purification:Immunoaffinity purification followed by ion-exchange chromatography.Presentation:10 mM potassium phosphate, pH 7.4, 150 mM NaClStorage Conditions:-70 °C (between -65 and -80 °C allowed)

Advanced ImmunoChemical, Inc. ANTI-PSA MAB

MONOCLONAL ANTIBODIES/MAbs in vitro:8A6ccMAbs in vivo:5A6, PS2, 1H12Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with human PSA antigen purified from seminal plasma.MAb Isotypes:IgG1 for MAbs PS2, IgG2a for MAbs 5A6, 8A6cc, 1H12Applications:Detection of PSA antigen. Recommended pairs for detection of total PSA (capture-detection):Recommended pairs for detection of free PSA (capture-detection):5A6-8A6cc, Best pair: 8A6cc – 5A6MAbs can also be used in lateral flow.Purification:MAbs PS2 by ion exchange chromatography and other MAbs by chromatography on protein A SepharosePresentation:MAbs 5A6, 8A6, and 1H12 in PBS, pH 7.4, 0.1 % sodium azide (NaN3) MAbs PS2 in 0.15 M NaCl, 10 mM Tris, pH 7.5, 0.05% sodium azide (NaN3)Storage Conditions:+ 4 °C

Advanced ImmunoChemical, Inc. Anti-RABIES VIRUS MAB

MONOCLONAL ANTIBODIES/MAbs in vitro:1C5ccMAbs in vivo:4G4, New MAbs: 4F1, 7E3Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified rabies virus. Specificity:MAbs 4G4, 4F1, 7E3 are specific to glycoprotein.MAb 1C5cc has a virus-neutralizing activity in respect to the majority of standard RV and rabies-relative viruses: CVS, Lagos bat, Mokola, Flury lep, Duvenhage and the field isolates.MAb Isotypes:IgG2a for MAbs 1C5ccIgG2b for MAbs 4G4, 4F1, 7E3Applications:MAbs 1C5cc, 4G4,4F1, 7E3 are working in EIA.MAbs 4F1, 7E3 are working in Western blotting.MAbs 4F1, 7E3 are working in immunofluorescence.MAb 1C5cc works in immunocytochemistry.Purification:MAb 1C5cc is purified by chromatography on protein G Sepharose and all other MAbs on protein A Sepharose.Presentation:PBS, pH 7.4, 0.1 % sodium azide (NaN3)Storage Conditions:+ 4 °C

Advanced ImmunoChemical, Inc. ANTI-INFLUENZA A HAEM H5

MONOCLONAL ANTIBODIES/MAbs:1C7, 1B4. Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified avian influenza virus, type A (H5N1).Specificity:MAbsreactwithhaemagglutininH5.Mouse Isotypes:1C7, 1B4 – IgG2aApplications:MAbs 1C7 can be used in haemagglutinin inhibition (HI) test. MAb 1B4 can be used in ELISA.Recommended pair for sandwich immunoassay (capture – detection): 1B4 – 1C7.Purification:Protein G chromatography.Presentation:PBS, pH 7.4, 0.09% sodium azide (NaN3).MAb: Concentration: mg/mlLot: Volume: mlExpiration date: Amount: mgStorage Conditions:Store @ +4oC (+2 ̊…+8 ̊C allowable).

Advanced ImmunoChemical, Inc. ANTI-HUMAN LEPTIN MAB

MONOCLONAL ANTIBODIES/Monoclonal mouse anti-human leptin. MAbs:3G7, 4F12Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with recombinant leptin.Specificity:MAbs recognize recombinant human leptin and leptin circulating in human blood. MAb Isotypes:IgG1 for all MAbs for all MAbsApplications:Leptin immunoassay. All MAbs recognize human leptin in Western blotting. MAbs were tested in sandwich immunoassay with human blood samples. All recommended pairs recognize antigen in human blood with high sensitivity. Recommended pairs for sandwich immunoassay (capture – detection): 3G7 – 4F124F12 – 3G7. Purification: MAb 4F12: Chromatography on protein A Sepharose and gel-filtration, other MAbs: Chromatography on protein A Sepharose. Presentation: PBS, pH 7.4, 0.1 % sodium azide (NaN3)Storage Conditions:+ 4 °C.

Advanced ImmunoChemical, Inc. ANTI-HUMAN HEMOGLOBIN HBA10

MONOCLONAL ANTIBODIES/MAbs:Hb4, Hb6Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with glycated (in vitro) synthetic peptide corresponding to the N-terminal end of beta-globin.Specificity:Native human HbA10MAb Isotypes:IgG1 for MAbs Hb4 and Hb6Applications:HbA1c immunoassay.Purification:MAb 4F12: Chromatography on protein A Sepharose and gel-filtration, other MAbs: Chromatography on protein A SepharosePresentation:PBS, pH 7.4, 0.1 % sodium azide (NaN3)Storage Conditions:+ 4 °C