HPLC Columns
Waters Corp WATERS CORPORATION Column, 80Å, 5 μm, 4.6 mm, 250 mm
Spherisorb SCX columns feature an anionic silica-based sorbent, providing strong cation-exchange for separating positively charged ionic species. These columns are engineered for high efficiency, optimal peak shape, and excellent reproducibility, ensuring a longer operational lifetime with a rugged silica particle design for enhanced durability.
- Ion-exchange separation mode with silica particle substrate.
- pH range of 2 to 8; maximum pressure of 6000 psi (415 bar).
- Spherical particle (5 μm) with 80 Å pore size for HPLC systems.
- 4.6 mm inner diameter; 250 mm length.
- cGMP, ISO 9002 certified plant manufacturing; individually tested.
- Enhances efficiency, peak shape, reproducibility, and lifetime.
Waters Corp Waters IC-Pak Anion Column, 10 μm, 4.6 mm ID, 50 mm Length
IC-Pak resin-based columns facilitate the analysis of a wide range of ions from simple to complex sample matrices. They offer an exceptional linear loading range (under 1.0 ppb to over 400 ppm) without dilution or pH limitations, ensuring accurate, reproducible anion and cation analyses across all concentration levels.
- Designed for inorganic anion analysis.
- Stainless steel column construction.
- Separates and quantifies a wide range of inorganic anions at ppb levels.
- Polymethacrylate resin packing with a quaternary ammonium functional group.
- Maximum pressure: 1000 psi (70 Bar).
- Particle size: 10 μm.
- Inner diameter: 4.6 mm.
- Length: 50 mm.
- Compatible with HPLC systems.
- Manufactured in a cGMP, ISO 9001 certified plant; individually tested with eCord™ Intelligent Chip for performance and batch analysis documentation.
Waters Corp Waters Ultrahydrogel DNA Column, 10 μm, 7.8 mm x 300 mm
Waters Ultrahydrogel Columns contain a sophisticated aqueous-compatible gel for analytical and preparative separations of water-soluble polymers. The gel is a cross-linked hydroxylated polymer with some residual carboxyl functionality. These columns are used to characterize oligomers and biological substances such as polysaccharides, nucleic acids, proteins, and peptides.
- High-resolution separations.
- Wide pH range (2 to 12 pH).
- Compatibility with high concentrations of organic solvents.
- Higher mobile phase flexibility.
- Lower incidence of non-size-exclusion effects.
- Manufactured under strict controls to ensure adherence to high industry standards.
Waters Corp Waters C18 Column, 15 μm, 100 Å, 3.9 mm × 300 mm
This C18 column is primarily used for legacy methods. It features a highly stable, bonded, endcapped 15 μm spherical silica packing, available in various cartridge and column configurations. It provides consistent and predictable scale-up capabilities for milligram-to-gram scale purification, and the availability of the same chemistry on a high-efficiency 5 μm particle allows for easy checking of fraction purity.
- Based on a highly stable, bonded, endcapped 15 μm spherical silica.
- Features 100 Å pore size and C18 bonded phase.
- Available in different cartridge and column configurations.
- Provides consistent and predictable scale-up capabilities for milligram-to-gram scale purification.
- Allows for easy purity checks of fractions when the same chemistry is available on a high-efficiency 5 μm particle.
Waters Corp XBridge BEH C8 Column, 130Å, 5 µm, 2.1 mm X 100 mm, 1/pk
The XBridge BEH C8 sorbent is less retentive compared to the XBridge BEH C18 sorbent. Anyone developing methods may prefer the speed offered by a less retentive column, while improving peak shape and chromatographic performance. The trifunctionally bonded sorbent offers you the widest usable pH range (1-12), superior low pH stability, and ultra-low column bleed.
Waters Corporation OBD Prep Column, BEH Shield RP18, 5 μm, 19 mm x 150 mm
These XBridge BEH Shield RP18 OBD Prep Columns are designed for HPLC method development and preparative liquid chromatography, offering unique selectivity and high performance.
- Provides alternate selectivity for phenolic compounds via an embedded carbamate group.
- Features reversed phase separation with hybrid, spherical BEH particles (5 μm, 130 Å pore).
- Stable across a pH range of 2 to 11 (max 50 °C low pH / 45 °C high pH), up to 6000 psi (415 Bar).
- Low silanol activity with Shield RP18 bonding and TMS endcap style.
- 19 mm inner diameter by 150 mm length OBD preparative column format.
- Uniform packed bed density ensures direct scalability from analytical to preparative methods.
- Excellent column lifetime and consistent performance deliver optimal resolution, peak shape, and mass loading.
Waters Corp SunFire C18 Method Validation Kit, 100Å, 5 µm, 4.6 mm X 150 mm, 3/pk
This kit consists of three Sunfire C18 columns packed with three different batches of sorbent.
Waters Corp SunFire C8 Column, 100Å, 5 µm, 2.1 mm X 100 mm, 1/pk
SunFire C8 columns are used for general purpose method development at low pH. The C8 ligand is preferred for some assays because it is less hydrophobic and therefore less retentive compared to SunFire C18.
Waters Corporation Column, C18, 3.5 μm, 2.1 mm × 20 mm
The XBridge BEH C18 sorbent functions as a workhorse for High-Performance Liquid Chromatography (HPLC) method development, usable across a mobile phase pH range of 1 to 12. Its trifunctionally bonded C18 ligand offers a universal column solution for most HPLC separations, providing a wide usable pH range, enhanced low pH stability, and minimal column bleed.
- Chemistry: C18
- Separation mode: Reversed phase
- Particle substrate: Hybrid
- pH range: 1 to 12
- Maximum pressure: 6000 psi (415 bar)
- Particle size: 3.5 μm
- Pore size: 130 Å
- Surface area: 185
- Inner diameter: 2.1 mm
- Length: 20 mm
- Carbon load: 18 %
- Units per package: 1 pk
Waters Corp XTerra Shield RP8 OBD Prep Column, 10 μm, 50 mm x 250 mm
XTerra Preparative RP8 columns are hybrid-based, reversed-phase C8 columns. They incorporate Waters patented embedded polar group technology to improve peak shape performance for highly basic compounds. These first-generation columns offer superior pH stability compared to silica-based columns.
- C8 chemistry and reversed phase separation mode
- Hybrid particle substrate
- pH range of 2 to 12
- Temperature limits up to 60 °C
- Maximum pressure of 6000 psi (415 Bar)
- Endcapped design for enhanced performance
- Shield RP8 bonding technology with low silanol activity
- Spherical particle shape with 10 μm particle size
- Pore size of 125 Å
- Inner diameter of 50 mm and length of 250 mm
- 14 % carbon load
- Suitable for HPLC preparative systems and LC/MS, LC techniques
- USP classification L7
Waters Corp Waters Column, XBridge BEH Shield RP18, 130Å, 5 μm, 4.6 mm x 20 mm
XBridge BEH Shield RP18 columns complement C18 and C8 phases, serving as a vital tool for HPLC method development. Their embedded carbamate group offers alternative selectivity, especially for phenolic compounds, compared to traditional alkyl columns.
- Chemistry: C18
- Separation mode: Reversed phase
- Particle substrate: Hybrid
- pH range: 2 to 11
- Maximum pressure: 6000 psi (415 Bar)
- Endcapped: Yes
- Bonding technology: Shield RP18
- Silanol activity: Low
- Particle shape: Spherical
- Particle size: 5 μm
- Pore size: 130 Å
- Format: Intelligent speed (IS) column
- USP classification: L1
- Inner diameter: 4.6 mm
- Length: 20 mm
- Carbon load: 17 %
Waters Corp Waters Column, HSS C18, 100Å, 1.8 μm, 1 mm X 100 mm
The ACQUITY UPLC HSS C18 chemistry is a general-purpose C18 bonded phase designed for ultra-performance liquid chromatography. It provides superior peak shape for bases, increased retention, and exceptionally long column lifetimes at low pH. These columns are fully scalable and transferable to HPLC separations.
- General-purpose C18 bonded phase for ultra-performance liquid chromatography
- Superior peak shape for bases and increased retention
- Exceptionally long column lifetimes at low pH
- Fully scalable and transferable to HPLC separations
- Chemistry: C18
- Separation mode: Reversed phase
- Particle substrate: Silica
- pH range: 1 to 8 pH
- Temperature limits: 45 °C
- Maximum pressure: 18000 psi (1240 Bar)
- Particle size: 1.8 μm
- Pore size: 100 Å
- Dimensions: 1 mm inner diameter X 100 mm length
Waters Corp Waters Preparative Column, XBridge BEH C18, 10 μm, 19 x 150 mm
The XBridge BEH C18 sorbent facilitates HPLC method development, usable across a mobile phase pH range of 1 to 12. Its trifunctionally bonded C18 ligand provides universal HPLC separations with superior low pH stability and ultra-low column bleed. The Optimum Bed Density (OBD) design enables faster, more effective lab-scale separations and direct scale-up from UPLC or HPLC to purification. This column resists mechanical chromatographic bed failure.
- Usable across a mobile phase pH range of 1 to 12.
- Trifunctionally bonded C18 ligand for universal HPLC separations.
- Superior low pH stability and ultra-low column bleed.
- Improved output and recovery.
- Faster, more effective lab-scale separations.
- Direct scale-up from UPLC or HPLC to lab-scale purification.
- Resistant to mechanical chromatographic bed failure.
- Consistent, predictable density profiles and cavity avoidance.
Waters Corp Waters XBridge Peptide BEH C18 Column, 5 μm, 130 Å, 2.1 mm × 150 mm
The XBridge Peptide BEH C18 columns are optimized and QC tested for peptide separations. Featuring a small-pore (130Å) trifunctionally bonded BEH particle, they offer a wide pH range (1 to 12), superior low pH stability, and ultra-low column bleed, making them ideal for proteomics, protein characterization, and peptide synthesis.
- Optimized and QC tested for peptide separations.
- Widest usable pH range (1 to 12 pH).
- Superior low pH stability and ultra-low column bleed.
- Provides highest level of quality and consistency due to synthetic particles.
- Manufactured in facilities adhering to cGMP and ISO 9001 standards.
- Reliable for consistent synthetic peptide and protein digest separations.