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Restriction enzymes, modifying enzymes, buffering solutions, inhibitors, and substrates for use in clinical, research, and general laboratory procedures.
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H-Glu(AMC)-OH is a fluorogenic amino acid derivative used as a peptide substrate to measure aminopeptidase A enzyme activity. Enzymatic cleavage releases 7-amino-4-methylcoumarin (AMC), producing a fluorescent signal that enables quantitative fluorescence-based activity assays. Intended for research use only.
Fluorogenic substrate for aminopeptidase A activity assays.
Releases 7-amino-4-methylcoumarin (AMC) upon enzymatic cleavage.
Ac-Arg-Leu-Arg-AMC (5 mg), AdipoGen Life Sciences. Chemical. CAS: 929903-87-7 (free base). Formula: C30H46N10O6 . C2HF3O2. MW: 642.8 . 114.0. Fluorogenic tri-peptide substrate for measuring the trypsin-like peptidase activity of the 20S proteasome. It is cleaved exclusively at the amide R-AMC bond to release fluorescent product. Shows low Km and high specific activity. Excitation: 380nm. Emission: 460nm. This substrate is useful for inhibitor screening and kinetic analysis.
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Boc-LRR-AMC (Boc-Leu-Arg-Arg-AMC) is a fluorogenic substrate which can be utilized for detecting the trypsin-like activity of the 26S proteasome or the 20S proteasome core[1]
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A highly sensitive, stable substrate for HRP that reacts with H2O2 to produce the fluorescent compound resorufin; enables detection of H2O2 at a concentration as low as 5 pmol per 100 µl samples
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Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This water soluble substrate for the firefly luciferase enzyme utilizes ATP and Mg2+ as cofactors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C. Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain.
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Ac-Trp-Leu-Ala-AMC [Ac-WLA-AMC] (2 mg), AdipoGen Life Sciences. Chemical. CAS: 1104011-59-7. Formula: C32H37N5O6. MW: 587.7. Synthetic. Ac-WLA-AMC is a fluorogenic peptid substrate for measuring chymotrypsin-like activity of the proteasome. Hydrolysis of this substrate by the beta5-subunit of the 20S proteasome is monitored by observing fluorescence at an Excitation wavelength of 345nm and Emission at 445nm. This substrate is specific to the constitutive proteasome and is not hydrolyzed efficiently by the immunoproteasome. 20S Proteasome enzyme requires activation with 0.035% SDS in the assay buffer.
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This peptide, KVEKIGEGTYGVVYK, is derived from amino acids 6-20 of cdc2 and is a good substrate for src and other tyrosine kinases. Alternative name: cdc2 (6-20). Purity: ≥95%. Long Term Storage: -20°C.
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An ideal alternative to other, more sensitive substrates where background is a problem. 4 CN (4-chloro-1-napthol) produces a precipitate at the reaction site in the presence of peroxidase-labeled conjugates. It is optimized for use in membrane assays and dot blots. 2-component format, simply mix and use.
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