Enzymes and Inhibitors
New England Biolabs, Inc. T4 Polynucleotide Kinase Reaction Buffer – 4 ml
New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme.
New England Biolabs, Inc. CpG Methyltransferase (M.SssI) – 500 units
The CpG Methyltransferase, M.SssI, methylates all cytosine residues (C5) within the double-stranded dinucleotide recognition sequence 5'...CG...3'
New England Biolabs, Inc. T7 Exonuclease – 5000 units
T7 Exonuclease acts in the 5' to 3' direction, catalyzing the removal of 5' mononucleotides from linear or nicked duplex DNA.
New England Biolabs, Inc. ShortCut RNase III – 200 units
ShortCut RNase III, used with its manganese-containing reaction buffer, converts long double-stranded RNA into a heterogeneous mix of short (18-25 bp) interfering RNAs (siRNA) suitable for RNA interference in mammalian cells. 1.5 units (1 µl) of ShortCut RNase III is sufficient to convert 1µ g of dsRNA into siRNA suitable for RNA interference in mammalian cells.
New England Biolabs, Inc. α1-6 Mannosidase - 800 units
a1-6 Mannosidase is a highly specific exoglycosidase that removes unbranched a1-6 linked mannose residues from oligosaccharides. When used in conjunction with a1-2,3 Mannosidase, the a1-6 Mannosidase will cleave a1-6 mannose residues from branched carbohydrate substrates.
New England Biolabs, Inc. Nt.BbvCI – 1000 units
A restriction endonuclease that recognizes the sequence CC^TCAGC.
New England Biolabs, Inc. DpnII – 5000 units
A restriction endonuclease that recognizes the sequence ^GATC_.
New England Biolabs, Inc. StyD4I – 200 units
A restriction endonuclease that recognizes the sequence ^CCNGG_.
New England Biolabs, Inc. BsaJI – 1000 units
A restriction endonuclease that recognizes the sequence C^CNNG_G.
New England Biolabs, Inc. MwoI – 2500 units
A restriction endonuclease that recognizes the sequence GCNN_NNN^NNGC.
New England Biolabs, Inc. NspI – 250 units
A restriction endonuclease that recognizes the sequence R_CATG^Y.
New England Biolabs, Inc. HpyCH4IV – 2500 units
A restriction endonuclease that recognizes the sequence A^CG_T.
New England Biolabs, Inc. BspCNI – 100 units
A restriction endonuclease that recognizes the sequence CTCAGNNNNNNN_NN^.
New England Biolabs, Inc. NotI – 2500 units
A restriction endonuclease that recognizes the sequence GC^GGCC_GC.