Modifying Enzymes
Alkaline Phosphatase, 2500 U, G-Biosciences
TAB5 Alkaline Phosphatase is a heat labile alkaline phosphatase. It catalyzes the dephosphorylation of phosphomonoesters at both 5' and 3' ends of DNA and RNA molecules in a nonspecific manner. Additionally, it eliminates phosphate groups from the 5' and 3' positions of DNA, RNA, and dNTPs.
E. coli Gyrase, 100 U, G-Biosciences
Gyrase is a type IA DNA topoisomerase and it is involved in bacterial DNA supercoiling.
E. coli Exonuclease I, 5000 U, G-Biosciences
Exonuclease I (Exo I), is a highly processive single-stranded DNA-specific exonuclease which removes mono- and dinucleotides from the 3' end of single-stranded DNA molecules (3' to 5' exonuclease activity) leaving double-stranded molecules and the 5' end of single stranded molecules intact.
G-Biosciences RNase Inhibitor (Murine)
RNase Inhibitor (Murine) is used for first strand cDNA synthesis, RT-qPCR.
Endonuclease VIII, 2000 U, G-Biosciences
Endonuclease VIII from E. coli has dual functions as an N-glycosylase and an AP-lyase. It removes damaged pyrimidines from double-stranded DNA to create an AP site. The AP-lyase activity then cleaves at this site, resulting in a 5′ phosphate and a 3′ phosphate.
Exonuclease III, 25000 U, G-Biosciences
Exonuclease III catalyzes the sequential excision of mononucleotides from 3'-hydroxyl termini of duplex DNA, or the RNA strand of a DNA/RNA hybrid.
Thermolabile UDG, 500 U, G-Biosciences
Thermolabile Uracil-DNA Glycosylase (UDG), originating from the species Gadus morhua, removes uracil from DNA by hydrolyzing the N-glycosylic bond between the deoxyribose and the base leaving an AP (apurinic or apyrimidinic) site.
G-Biosciences T4 Polynucleotide Kinase (PNK)
T4 Polynucleotide Kinase (PNK) exhibits 5' polynucleotide kinase and 3' phosphatase activity.
RNase R, 500 U, G-Biosciences
A C-terminal truncated version of the E.coli RNase R. This truncated enzyme exhibits higher specific activity compared to the full length enzyme.
T4 RNA ligase II, 750 U, G-Biosciences
T4 RNA Ligase II (gp24.1) repairs nicks in dsRNA and can also be used to ligate the 3' terminal OH of RNA to the 5'-terminal phosphate of DNA in a double-stranded structure.
G-Biosciences T5 Exonuclease
T5 Exonuclease is a highly efficient 5'-to-3' exonuclease for either ssDNA or dsDNA. It also has ssDNA specific endonuclease activity in the presence of magnesium ions.
Klenow Exo, 1000 U, G-Biosciences
Klenow fragment (Exo-) is a mesophilic DNA polymerase deficient in both proofreading (3′→5′) and nick-translation (5′→3′) nuclease activities, with a moderate strand displacement activity during DNA synthesis.
Terminal deoxynucleotidyl transferase (TdT), Murine, 2500 U, G-Biosciences
Terminal Transferase catalyzes the untemplated addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules.
T7 Exonuclease, 2500 U, G-Biosciences
Gene 6 protein of bacteriophage T7 is a dsDNA 5' to 3' exonuclease which also exhibits flap endonuclease activity.