Restriction Enzymes
New England Biolabs, Inc. Tth111I – 400 units
A restriction endonuclease that recognizes the sequence GACN^N_NGTC.
New England Biolabs, Inc. XcmI – 5000 units
A restriction endonuclease that recognizes the sequence CCANNNN_N^NNNNTGG.
New England Biolabs, Inc. PleI – 1000 units
A restriction endonuclease that recognizes the sequence GAGTCNNNN^N_.
New England Biolabs, Inc. NarI – 500 units
A restriction endonuclease that recognizes the sequence GG^CG_CC.
New England Biolabs, Inc. Hpy166II – 1000 units
A restriction endonuclease that recognizes the sequence GTN^_NAC.
New England Biolabs, Inc. Nb.BtsI – 1000 units
A restriction endonuclease that recognizes the sequence GCAGTG_NN.
New England Biolabs, Inc. EaeI – 200 units
A restriction endonuclease that recognizes the sequence Y^GGCC_R.
New England Biolabs, Inc. PI-SceI – 250 units
A restriction endonuclease that recognizes the sequence ATCTATGTCGG_GTGC^GGAGAAAGAGGTAAT. The intein encoding PI-SceI is present in the VMA ATPase gene Saccharomyces cerevisiae. The gene has been modified for independent expression in E.coli using a T7 RNA polymerase expression system.
New England Biolabs, Inc. EcoGII Methyltransferase – 200 units
EcoGII Methyltransferase is a non-specific methyltransferase that modifies adenine residues (N6) in any sequence context.
INTACT GENOMICS INC PEmax Enzyme 80 µg
Intact Genomics (IG) PEmax enzyme is the purified recombinant Streptococcus pyogenes Cas9 nickase mutant (H840A)-Murine Moloney Leukemia Virus-Reverse Transcriptase (MMLV-RT) fusion protein3-4 containing a nuclear localization signal (NLS) at the C-terminal for targeting to the nucleus. This enzyme is designed to perform CRISPR prime-editing3-4. The physical purity of this enzyme is ≥85% as assessed by SDS-PAGE with Coomassie blue staining.Product Includes: PEmax enzyme 5x PEmax Nicking Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM MgCl2, 0.1 mM EDTA, pH 6.5 @ 25 °CStore all components at -20°C.
New England Biolabs, Inc. SphI-HF – 500 units
A high fidelity restriction endonuclease that recognizes the sequence GCATG^C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
New England Biolabs, Inc. ApoI-HF – 1000 units
A high fidelity restriction endonuclease that recognizes the sequence R^AATTY. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
Cayman Chemical NItrate Reductase Enzyme Prep
A VEGFR1 VEGFR2 and EGFR inhibitor (IC50s 0.7 0.03 and 0.4 UM respectively) selective for these kinases over PDGFRB FGFR1 and HER2 (IC50s 3.4 >100 and >100 UM respectively) and MEK Cdk2 IGF-1R Akt and Tie2 (IC50s >10 >10 >20 >20 and >100 UM respectively) selectively inhibits VEGF- or EGF-induced proliferation of HUVECs over FGF-induced proliferation of HUVECs (IC50s 0.05 0.05 and 1.5 UM respectively) reduces tumor volume in a Calu-6 NSCLC mouse xenograft model at 100 mg/kg inhibits training-induced increases in collateral blood flow and white gastrocnemius muscle capillarity in exercise-trained rats
New England Biolabs, Inc. Nb.BssSI – 1000 units
A restriction endonuclease that recognizes the sequence CACGAG.