Cell Culture Media, Supplements, and Reagents
Lonza Walkersville MycoAlert Mycoplasma Detection Kit (100 Tests)
Lonza’s MycoAlert Mycoplasma Detection Assays are selective biochemical tests to detect Mycoplasma contamination in cell cultures. They exploit the activity of mycoplasmal enzymes which are found in the vast majority of about 200 mycoplasma species, but are not present in eukaryotic cells. Benefits:Convenient & fastSimply add two reagents to the culture supernatantPerform two luminescence readingsResults in less than 20 minEasy interpretation of results Detects all common mollicute contaminations (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma except Ureaplasma)Negligible interference with media componentsContent:1 x 10 mL MycoAlert Reagent (lyophilized)1 x 10 mL MycoAlert Substrate (lyophilized)1 x 20 mL MycoAlert Assay Buffer
STEMCELL Technologies ImmunoCult™ Human CD3/CD28 T Cell Activator
Achieve robust activation and expansion of T cells in the absence of magnetic beads, feeder cells, or antigens.This product’s gentle activation stimulus ensures a high viability of activated T cells, which can be further expanded in ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981) or other media for culturing human T cells. 1. Antibody complexes bind to and cross-link CD3 and CD28 cell surface ligands, thereby providing the required signals for T cell activation. ImmunoCult™ Human CD3/CD28 T Cell Activator can be used on the Seahorse XF Analyzer to measure T cell activation response and is also available as part of the Agilent Seahorse XF Hu T Cell Activation Assay Kit.This product is designed for cell therapy research applications, but may be qualified for use as an ancillary material following the framework outlined in USP. STEMCELL can work with you to qualify this reagent as an AM under an approved investigational new drug or clinical trial application.
STEMCELL Technologies ACCUTASE™, Size: 100 mL
A cell detachment solution of proteolytic and collagenolytic enzymes. Useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware. ACCUTASE™ does not contain mammalian or bacterial-derived products. Each lot of ACCUTASE™ is tested for sterility (by USP membrane filtration method), enzymatic activity (tested with synthetic chromagenic tetrapeptides) and cell detachment from tissue culture plastic.
STEMCELL Technologies ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator, Size: 2 mL
Achieve robust activation and expansion of T cells in the absence of magnetic beads, feeder cells, or antigens. This product’s gentle activation stimulus ensures a high viability of activated T cells, which can be further expanded in ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981) or other media for culturing human T cells. ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator consists of soluble tetrameric antibody complexes that bind to and cross-link CD3, CD28, and CD2 cell surface ligands, thereby providing the required primary and co-stimulatory signals for T cell activation. This product is designed for cell therapy research applications, but may be qualified for use as an ancillary material (AM) following the framework outlined in USP<1043>. Contact STEMCELL Technologies to learn how we can work with you to qualify this reagent as an AM under an approved investigational new drug (IND) or clinical trial application (CTA).
Vector Laboratories VECTASHIELD PLUS Antifade Mounting Medium with DAPI | 10mL
VECTASHIELD PLUS Antifade Mounting Medium with DAPI is a unique, stable formula for preserving fluorescence. This medium inhibits rapid photobleaching of fluorescent proteins and fluorescent dyes.
STEMCELL Technologies 0.1% Gelatin in Water, Size: 500 mL
This product is recommended for the coating of culture dishes or flasks to grow mouse embryonic stem cells (mESCs), mouse induced pluripotent stem cells (miPSCs) or primary embryonic fibroblasts (PEFs).
Invivogen Zeocin Selective antibiotic for the Sh ble gene, 1g (10 x 1 ml)
Zeocin, 1 g Liquid @ 100 mg/mL, Color: Blue, Storage: -20 deg.C, Purity: 90% > HPLC and Microbiological Assays, Packaging: 5 x 2 mL Tubes, pH: 7.25, CAS No: 11006-33-0
Iwai North America CELLBANKER 2: Ready to use serum free cryopreservation media. Size: 100mL
CELLBANKER 2 is a part of the renowned CELLBANKER series, cited in over 1400 publications. As part of Japan's most trusted line of cryopreservation media, it is serum free, is ready to use, and requires no liquid nitrogen for cell preservation. With over a decade of data and extensive testing on diverse cell lines, tissues, and organoids, trust CELLBANKER 2 for safe, hassle-free cell storage.
FUJIFILM BIOSCIENCES INC BalanCD HEK293 Feed
BalanCD HEK293 Feed is formulated to significantly increase protein yields in suspension-adapted HEK293 cellLines post-transfection.
Sciencell Research Laboratories Astrocyte Medium, Complete Kit (includes supplements)
Astrocyte medium is a complete medium designed for optimal growth of normal human astrocytes in vitro. It is shipped as a kit with FBS, astrocyte growth supplement, and Pen/Strep solution.
STEMCELL Technologies DNase I Solution (1 mg/mL), Size: 1 mL
Deoxyribonuclease (DNase) I Solution (1 mg/mL) is useful to reduce or prevent the clumping of concentrated and/or cryopreserved cell suspensions following thawing. The activity range is ≥ 2000 units per mg of protein.
STEMCELL Technologies Lymphoprep™, Size: 500 mL
Reliably isolate mononuclear cells from peripheral blood, cord blood, or bone marrow with Lymphoprep™—a cost-effective alternative to Ficoll-Paque™. Use this density gradient medium for rapid, simple, and reliable cell isolation from most blood samples obtained from normal individuals and patients. You can substitute Lymphoprep™ for Ficoll-Paque™ without changing your existing protocols and achieve similar cell purity and recovery rates. This medium is fully compatible with both SepMate™ and RosetteSep™. Formulated with sodium diatrizoate (9.1% w/v) and polysaccharide (5.7% w/v), Lymphoprep™ has a density of 1.077 g/ml. Visit STEMCELL.com/lymphoprep for more information and to see the performance data.
STEMCELL Technologies PneumaCult™-ALI Medium
PneumaCult™-ALI Medium (Catalog #05001) is a serum- and BPE-free medium for the culture of human airway epithelial cells at the air-liquid interface (ALI). Airway epithelial cells cultured in PneumaCult™-ALI Medium undergo extensive mucociliary differentiation to form a pseudostratified epithelium that exhibits morphological and functional characteristics similar to those of the human airway in vivo. PneumaCult™-ALI Medium is also available in a kit that includes 12 mm Transwell inserts (Catalog #05021) or 6.5 mm Transwell inserts (Catalog #05022).Together, PneumaCult™-ALI Medium and PneumaCult™-Ex Plus Medium (Catalog #05040) constitute a fully integrated BPE-free culture system for in vitro human airway modeling that is also compatible with primary human nasal epithelial cells. This robust and defined system is a valuable tool for basic respiratory research, toxicity studies, and drug development.
Invivogen FUNGIN 75 MG (10 MG/ML)
Fungin™ kills fungi and yeast by interfering with the cell membrane. The ergosterol, only found in fungi and yeast, is the primary site of action of Pimaricin. This polyene-induced distortion of selective membrane permeability results in a leakage of potassium and magnesium ions and a decrease of macromolecule synthesis. The death of fungi and yeast cells is characterized by the penetration of protons resulting in intracellular acidification. Product concentration: 10 mg/ml Formula: C33H47NO13 Molecular weight: 665.74 Active concentration: from 10 μg/ml to 50 μg/ml Highly stable and water soluble compound Compatible with Pen/Strep solutions No toxicity on treated cells and no deleterious effects on cell metabolism