Advanced ImmunoChemical, Inc.

Advanced ImmunoChemical, Inc. CA-125, Source - Human Adenocarcinoma, 250 KIU

This item is manufactured by HyTest, Ltd and distributed by Advanced ImmunoChemical, Inc. Antigens / Tumor Markers / CA-125. Source:Human adenocarcinoma.Blood sample from the tissue donor was tested and found negative for HBsAg, HIV-1 and HIV-2 antibodies, HCV, and syphilis.Applications:Immunogen, in vitro studies.Immunological specificity:Immunological specificity confirmed by reaction with monoclonal antibody that is specific for CA-125.Presentation:Presentation: PBS, pH 7.4, 3 % sucrose, 0.05 % sodium azide (NaN3).Storage Conditions:-20 °C (between -15 and -30 °C allowed)Material safety note:This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Advanced ImmunoChemical, Inc. Canine Parvovirus Vp2 Protein, Recombinant, 0.1mg

This item is manufactured by HyTest, Ltd and distributed by Advanced ImmunoChemical, Inc.

Antigens / Veterinary / Recombinant canine parvovirus VP2. Expressed in an insect cell line Description: Recombinant canine parvovirus variant 2b (CPV-2b) capsid protein VP2 consists of 584 amino acid residues. Theoretical molecular weight 64657 Da. Theoretical pI 5.36. Does not contain any affinity tags. Applications: Calibrator or standard in immunoassays for CPV immunodetection or antigen in immunoassays for determination of CPV antibody titre in canine blood. Analysis: Purity > 90% (SDS-PAGE in reducing conditions) Concentration is determined spectrophotometrically. Immunoreactivity is confirmed by reaction with monoclonal antibodies (Cat. #3PV16) specific to canine parvovirus. Presentation: Lyophilized from 20 mM Na-phosphate, pH 7.4, 30 mM NaCl, 0.01% Tween 20, 10% trehalose. It is recommended to reconstitute this product with deionized water to its initial concentration.

Advanced ImmunoChemical, Inc. CA72-4, Source - Huma Metastatic Liver Carcinoma, 250 kIU

This item is manufactured by HyTest, Ltd and distributed by Advanced ImmunoChemical, Inc. Antigens / Tumor Markers / CA72-4. Source: Human metastatic liver carcinoma.Blood sample from the tissue donor was tested and found negative for HBsAg, HIV-1 and HIV-2 antibodies and HCV.Storage: -20 °C (-15 … -30 °C allowed). Applications: Immunogen, calibrator in immunoassays, in vitro studies.Presentation: PBS, pH 7.4, 0.05 % of sodium azide (NaN₃).Material safety note: This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material. Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Advanced ImmunoChemical, Inc. ANTI-RAT PROINSULIN MAB

MONOCLONAL ANTIBODIES/Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with fragment of rat proinsulin conjugated with a carrier protein.Specificity:Rat proinsulinMAb Isotypes:IgG1 for MAb CCI-17Applications:MAb recognizes rat proinsulin in ELISA and Sandwich type immunoassay. MAb binds specifically to proinsulin and does not bind to insulin or C-peptide. See our “Rat C-peptide and proinsulin” Assay Notes for more information. For sandwich immunoassay it is recommended to use MAb in pair with MAbs specific to N-terminal part of C-peptides I and II (see also the data sheet for Cat.# 1-CP-r and “Rat C-peptide and proinsulin” Assay Notes for more information).The recommended pair for sandwich-type rat proinsulin I and II immunoassay is (capture- detection): CCI-17 – CII-55 Purification:Chromatography on protein A SepharosePresentation:Presentation: PBS, pH 7.4, 0.1 % sodium azide (NaN3)

Advanced ImmunoChemical, Inc. Human Papillomavirus L1 Protein (HPVL1), Recombinant, Type 18, Analysis:Purity > 90 %

This item is manufactured by HyTest, Ltd and distributed by Advanced ImmunoChemical, Inc. Antigens/ Infectious Diseases / Human Papillomavirus L1 protein (HPVL1), recombinant, type 18. Analysis:Purity > 90 % (SDS-PAGE).Each lot is tested for protein content (Lowry method), lipid content (Total lipid), purity (SDS-PAGE), carbohydrate content (Anthrone method), antigen content (Immunoblot).Immunological activity:Tested in quantitative immunoblot with anti-HPVL1 antibodies.Purification:Purification is conducted from broken yeast cells by following scheme: clarification, ammonium sulfate precipitation, ultrafiltration, cation exchange chromatography, zonal centrifugation, size-exclusion chromatography, sterile filtration.Presentation:Presentation: 20 mM KH2PO4 (pH 7.2), 500 mM NaCl, 1.7 mM EDTA, 0.01% Tween-80, 1 mM 2-MercaptoethanoStorage Conditions:+ 4 °C (between +2 and +8 °C allowed)Material safety note:This product is sold for research use only.

Advanced ImmunoChemical, Inc. ANTI-PSA MAB

MONOCLONAL ANTIBODIES/MAbs in vitro:8A6ccMAbs in vivo:5A6, PS2, 1H12Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with human PSA antigen purified from seminal plasma.MAb Isotypes:IgG1 for MAbs PS2, IgG2a for MAbs 5A6, 8A6cc, 1H12Applications:Detection of PSA antigen. Recommended pairs for detection of total PSA (capture-detection):Recommended pairs for detection of free PSA (capture-detection):5A6-8A6cc, Best pair: 8A6cc – 5A6MAbs can also be used in lateral flow.Purification:MAbs PS2 by ion exchange chromatography and other MAbs by chromatography on protein A SepharosePresentation:MAbs 5A6, 8A6, and 1H12 in PBS, pH 7.4, 0.1 % sodium azide (NaN3) MAbs PS2 in 0.15 M NaCl, 10 mM Tris, pH 7.5, 0.05% sodium azide (NaN3)Storage Conditions:+ 4 °C

Advanced ImmunoChemical, Inc. ANTI-CYSTATIN C MAB

MONOCLONAL ANTIBODIES/MAbs:Cyst10, Cyst13, Cyst16, Cyst18, Cyst23, Cyst28New MAbs: Cyst11, Cyst20, Cyst29Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with cystatin C purified from human urine.Specificity:Cystatin C.MAb Isotypes:IgG1 for MAbs Cyst11, Cyst13, Cyst16, Cyst18, Cyst20, Cyst23, and Cyst28 IgG2a for MAb Cyst29 IgG3 for MAb Cyst10Applications:Cystatin C immunoassay, human cystatin C sandwich immunoassay. Recommended pairs for sandwich immunoassay (capture-detection):Cyst28Cyst23 – Cyst13Recommended pairs with the best cross-reaction with animal sera (capture-detection):Cyst11-Cyst20 (dog serum)Cyst29-Cyst16 (cat serum)Cyst29-Cyst20 (cat serum)Cyst29-Cyst20 (horse serum)Cyst20-Cyst13 (horse serum)Purification:Chromatography on protein A SepharosePresentation:PBS, pH 7.4, 0.1 % sodium azide (NaN3)Storage Conditions:+ 4 °C

Advanced ImmunoChemical, Inc. ANTI-KIDNEY INJURY MOLECULE

MONOCLONAL ANTIBODIES/MAbs:KIM70, KIM75Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with human recombinant KIM-1 ectodomain expressed in baculovirus system.Specificity:Human KIM-1MAb Isotypes:IgG1 for MAbs KIM70, KIM75Applications:MAbs KIM70, KIM75 react with human recombinant KIM-1 in sandwich ELISA and Western blotting.Recommended pairs KIM-1 immunodetection in sandwich immunoassay (capture-detection):KIM70 – KIM75Purification:Chromatography on protein A SepharosePresentation:PBS, pH 7.4, 0.1 % sodium azide (NaN3)Storage Conditions:+ 4 °C

Advanced ImmunoChemical, Inc. ANTI-HUMAN LEPTIN MAB

MONOCLONAL ANTIBODIES/Monoclonal mouse anti-human leptin. MAbs:3G7, 4F12Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with recombinant leptin.Specificity:MAbs recognize recombinant human leptin and leptin circulating in human blood. MAb Isotypes:IgG1 for all MAbs for all MAbsApplications:Leptin immunoassay. All MAbs recognize human leptin in Western blotting. MAbs were tested in sandwich immunoassay with human blood samples. All recommended pairs recognize antigen in human blood with high sensitivity. Recommended pairs for sandwich immunoassay (capture – detection): 3G7 – 4F124F12 – 3G7. Purification: MAb 4F12: Chromatography on protein A Sepharose and gel-filtration, other MAbs: Chromatography on protein A Sepharose. Presentation: PBS, pH 7.4, 0.1 % sodium azide (NaN3)Storage Conditions:+ 4 °C.

Advanced ImmunoChemical, Inc. Recombinant canine parvovirus VP2

Recombinant canine parvovirus variant 2b (CPV-2b) capsid protein VP2 consists of 584 amino acid residues. Theoretical molecular weight 64657 Da. Theoretical pI 5.36. Does not contain any affinity tags.

Advanced ImmunoChemical, Inc. ANTI-INFLUENZA A HAEM H5

MONOCLONAL ANTIBODIES/MAbs:1C7, 1B4. Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified avian influenza virus, type A (H5N1).Specificity:MAbsreactwithhaemagglutininH5.Mouse Isotypes:1C7, 1B4 – IgG2aApplications:MAbs 1C7 can be used in haemagglutinin inhibition (HI) test. MAb 1B4 can be used in ELISA.Recommended pair for sandwich immunoassay (capture – detection): 1B4 – 1C7.Purification:Protein G chromatography.Presentation:PBS, pH 7.4, 0.09% sodium azide (NaN3).MAb: Concentration: mg/mlLot: Volume: mlExpiration date: Amount: mgStorage Conditions:Store @ +4oC (+2 ̊…+8 ̊C allowable).

Advanced ImmunoChemical, Inc. PROCALCITONIN, HU RECOMBINANT

ANTIGENS/Source:E. coli expression systemDescription:Consists of a polypeptide corresponding to 1-116 amino acid residues of human procalcitonin. Does not contain any tags.Applications:An antigen for immunodetection of human PCTAnalysis:Purity > 95 %Immunoreactivity of recombinant human PCT is checked in sandwich immunoassay with specific monoclonal antibodies.Purification:Affinity and reverse-phase chromatographyPresentation:Lyophilized from 20 mM Tris-HCl, pH 8.0, 150 mM NaCl.Product is recommended to reconstitute with deionized water.Storage Conditions:Before and after reconstitution at -20 °C (between -15 and -30 °C allowed)

Advanced ImmunoChemical, Inc. ANTI-CA-19-9 MAB

MONOCLONAL ANTIBODIES/Monoclonal antibodies / Tumor Markers / Monoclonal mouse anti-CA19-9. MAbs:X91 Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with affinity purified human CA19-9 antigen. Specificity: CA19-9 antigen. MAb Isotypes: IgM for MAb X91Applications: Detection of CA19-9 antigen in immunoassays. MAbs are working in immunohistochemistry. Purification: Ion exchange chromatography. Presentation: 0.15 M NaCl, 10 mM Tris, pH 7.5, 0.05% sodium azide (NaN3). Storage Conditions:+ 4 °C.

Advanced ImmunoChemical, Inc. ANTI-HUMAN GLYCATED HBA1C MAB

MONOCLONAL ANTIBODIES/MAbs:75C9Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with glycated (in vitro) synthetic peptide corresponding to the N-terminal end of beta-globin.Specificity:Native human HbA1c, does not cross-react with non-glycated hemoglobin.MAb Isotypes:IgG1 for MAb 75C9Applications:HbA1c immunoassaysPurification:Chromatography on protein A SepharosePresentation:PBS, pH 7.4, 0.1 % sodium azide (NaN3)Storage Conditions:+ 4 °C

Advanced ImmunoChemical, Inc. ANTI-PROCALCITONIN MAB

MONOCLONAL ANTIBODIES/MAbs in vivo:6F10, 38F11, 44D9, 22A11, 18B7Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cellsof Balb/c mice immunized with fragments of human PCT conjugated with carrier protein.MAbs in vitro:27A3cc, 42cc, 14C12cc, P123, P124, P135, P160Specificity:MAb Isotypes:IgG1 for MAbs 6F10, 38F11, 22A11, 14C12cc, 18B7, P123, P124, P160IgG2a for MAbs 27A3cc, 42cc, 44D9, P135 Applications:MAbs 27A3cc, 42cc, 14C12cc, 44D9, 6F10, 38F11, 22A11, 18B7 are working in Western blotting.Purification:Protein A chromatographyPresentation:PBS, pH 7.4, 0.09 % sodium azide (NaN₃)Storage Conditions:+4 °C (+2 … +8 °C allowed)