Advanced ImmunoChemical, Inc.

Advanced ImmunoChemical, Inc. CA15-3, Standard Grade, Human Milk, 250 KIU

This item is manufactured by HyTest, Ltd and distributed by Advanced ImmunoChemical, Inc. Antigens / Tumor Markers / CA15-3, standard grade. Source:Human milk.Blood sample from the donor was tested and found negative for HBsAg, HIV-1 and HIV-2 antibodies, HCV, and syphilis.Applications:Calibrator in immunoassays, in vitro studies.Purification:High-speed centrifugation, salt precipitation and delipidization.Presentation:Solution in 1 M NaCl, 1 % Triton X 100, containing 0.09 % of sodium azide as preservative.Storage Conditions:Short-term storage (< 12 months) at +4 °C (between +2 and +8 °C allowed)Long-term storage at -20 °C (between -15 and -30 °C allowed)Material safety note:This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Advanced ImmunoChemical, Inc. Canine Parvovirus Vp2 Protein, Recombinant, 0.1mg

This item is manufactured by HyTest, Ltd and distributed by Advanced ImmunoChemical, Inc.

Antigens / Veterinary / Recombinant canine parvovirus VP2. Expressed in an insect cell line Description: Recombinant canine parvovirus variant 2b (CPV-2b) capsid protein VP2 consists of 584 amino acid residues. Theoretical molecular weight 64657 Da. Theoretical pI 5.36. Does not contain any affinity tags. Applications: Calibrator or standard in immunoassays for CPV immunodetection or antigen in immunoassays for determination of CPV antibody titre in canine blood. Analysis: Purity > 90% (SDS-PAGE in reducing conditions) Concentration is determined spectrophotometrically. Immunoreactivity is confirmed by reaction with monoclonal antibodies (Cat. #3PV16) specific to canine parvovirus. Presentation: Lyophilized from 20 mM Na-phosphate, pH 7.4, 30 mM NaCl, 0.01% Tween 20, 10% trehalose. It is recommended to reconstitute this product with deionized water to its initial concentration.

Advanced ImmunoChemical, Inc. CA19-9, Human Metastatic Liver Carcinoma, 250 KIU

This item is manufactured by HyTest, Ltd and distributed by Advanced ImmunoChemical, Inc. Antigens / Tumor Markers / CA19-9. Source:Human metastatic liver carcinoma.Blood sample from the tissue donor was tested and found negative for HBsAg, HIV-1 and HIV-2 antibodies, HCV, and syphilis.Applications:Immunogen, calibrator in immunoassays, in vitro studies.Specific activity:Specific activity confirmed by enzyme immunoassay.Presentation:PBS, pH 7.4, 3 % sucrose, 0.05 % sodium azide (NaN3).Storage Conditions:-20 °C (between -15 and -30 °C allowed)Material safety note:This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Advanced ImmunoChemical, Inc. ANTI-HUMAN LEPTIN MAB

MONOCLONAL ANTIBODIES/Monoclonal mouse anti-human leptin. MAbs:3G7, 4F12Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with recombinant leptin.Specificity:MAbs recognize recombinant human leptin and leptin circulating in human blood. MAb Isotypes:IgG1 for all MAbs for all MAbsApplications:Leptin immunoassay. All MAbs recognize human leptin in Western blotting. MAbs were tested in sandwich immunoassay with human blood samples. All recommended pairs recognize antigen in human blood with high sensitivity. Recommended pairs for sandwich immunoassay (capture – detection): 3G7 – 4F124F12 – 3G7. Purification: MAb 4F12: Chromatography on protein A Sepharose and gel-filtration, other MAbs: Chromatography on protein A Sepharose. Presentation: PBS, pH 7.4, 0.1 % sodium azide (NaN3)Storage Conditions:+ 4 °C.

Advanced ImmunoChemical, Inc. ANTI-HUMAN HEMOGLOBIN HBA10

MONOCLONAL ANTIBODIES/MAbs:Hb4, Hb6Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with glycated (in vitro) synthetic peptide corresponding to the N-terminal end of beta-globin.Specificity:Native human HbA10MAb Isotypes:IgG1 for MAbs Hb4 and Hb6Applications:HbA1c immunoassay.Purification:MAb 4F12: Chromatography on protein A Sepharose and gel-filtration, other MAbs: Chromatography on protein A SepharosePresentation:PBS, pH 7.4, 0.1 % sodium azide (NaN3)Storage Conditions:+ 4 °C

Advanced ImmunoChemical, Inc. ANTI-CYSTATIN C MAB

MONOCLONAL ANTIBODIES/MAbs:Cyst10, Cyst13, Cyst16, Cyst18, Cyst23, Cyst28New MAbs: Cyst11, Cyst20, Cyst29Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with cystatin C purified from human urine.Specificity:Cystatin C.MAb Isotypes:IgG1 for MAbs Cyst11, Cyst13, Cyst16, Cyst18, Cyst20, Cyst23, and Cyst28 IgG2a for MAb Cyst29 IgG3 for MAb Cyst10Applications:Cystatin C immunoassay, human cystatin C sandwich immunoassay. Recommended pairs for sandwich immunoassay (capture-detection):Cyst28Cyst23 – Cyst13Recommended pairs with the best cross-reaction with animal sera (capture-detection):Cyst11-Cyst20 (dog serum)Cyst29-Cyst16 (cat serum)Cyst29-Cyst20 (cat serum)Cyst29-Cyst20 (horse serum)Cyst20-Cyst13 (horse serum)Purification:Chromatography on protein A SepharosePresentation:PBS, pH 7.4, 0.1 % sodium azide (NaN3)Storage Conditions:+ 4 °C

Advanced ImmunoChemical, Inc. ANTI-INFLUENZA A HAEM H5

MONOCLONAL ANTIBODIES/MAbs:1C7, 1B4. Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified avian influenza virus, type A (H5N1).Specificity:MAbsreactwithhaemagglutininH5.Mouse Isotypes:1C7, 1B4 – IgG2aApplications:MAbs 1C7 can be used in haemagglutinin inhibition (HI) test. MAb 1B4 can be used in ELISA.Recommended pair for sandwich immunoassay (capture – detection): 1B4 – 1C7.Purification:Protein G chromatography.Presentation:PBS, pH 7.4, 0.09% sodium azide (NaN3).MAb: Concentration: mg/mlLot: Volume: mlExpiration date: Amount: mgStorage Conditions:Store @ +4oC (+2 ̊…+8 ̊C allowable).

Advanced ImmunoChemical, Inc. ANTI-PSA MAB

MONOCLONAL ANTIBODIES/MAbs in vitro:8A6ccMAbs in vivo:5A6, PS2, 1H12Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with human PSA antigen purified from seminal plasma.MAb Isotypes:IgG1 for MAbs PS2, IgG2a for MAbs 5A6, 8A6cc, 1H12Applications:Detection of PSA antigen. Recommended pairs for detection of total PSA (capture-detection):Recommended pairs for detection of free PSA (capture-detection):5A6-8A6cc, Best pair: 8A6cc – 5A6MAbs can also be used in lateral flow.Purification:MAbs PS2 by ion exchange chromatography and other MAbs by chromatography on protein A SepharosePresentation:MAbs 5A6, 8A6, and 1H12 in PBS, pH 7.4, 0.1 % sodium azide (NaN3) MAbs PS2 in 0.15 M NaCl, 10 mM Tris, pH 7.5, 0.05% sodium azide (NaN3)Storage Conditions:+ 4 °C

Advanced ImmunoChemical, Inc. Anti-RABIES VIRUS MAB

MONOCLONAL ANTIBODIES/MAbs in vitro:1C5ccMAbs in vivo:4G4, New MAbs: 4F1, 7E3Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified rabies virus. Specificity:MAbs 4G4, 4F1, 7E3 are specific to glycoprotein.MAb 1C5cc has a virus-neutralizing activity in respect to the majority of standard RV and rabies-relative viruses: CVS, Lagos bat, Mokola, Flury lep, Duvenhage and the field isolates.MAb Isotypes:IgG2a for MAbs 1C5ccIgG2b for MAbs 4G4, 4F1, 7E3Applications:MAbs 1C5cc, 4G4,4F1, 7E3 are working in EIA.MAbs 4F1, 7E3 are working in Western blotting.MAbs 4F1, 7E3 are working in immunofluorescence.MAb 1C5cc works in immunocytochemistry.Purification:MAb 1C5cc is purified by chromatography on protein G Sepharose and all other MAbs on protein A Sepharose.Presentation:PBS, pH 7.4, 0.1 % sodium azide (NaN3)Storage Conditions:+ 4 °C

Advanced ImmunoChemical, Inc. ANTI-HUMAN INSULIN MAB

Monoclonal antibodies / Metabolic Syndrome / Monoclonal mouse anti-human insulin. MAbs:C7C9, D4B8, 7F8, 3A6, 8E2, 7F5. Hybridoma clones have been derived from hybridization of X63-Ag8-653 myeloma cells with spleen cells of Balb/c mice immunized with purified human insulin.Specificity: Insulin, human; MAb C7C9 reacts with C-terminal pentapeptide of insulin β-chain. MAbs 7F8, D4B8, 3A6 and 8E2 react with different antigenic determinants. Kass for MAb 7F8 is 7.5 x 109M-1; Kass for MAb D4B8 is 1.5 x 108M-1; Kass for MAb 3A6 is 8.0 x 108M-1; Kass for MAb 8E2 is 6.0 x 108M-1; Kass for MAb C7C9 is 1.0 x 109M-1; Kass for MAb 7F5 is 1.0 x 109M-1. These antibodies cross-react with human proinsulin, bovine insulin (30%) and porcine insulin. No cross-reaction with free C-peptide. MAb isotypes: IgG1, kappa for all MAbs applications: Detection of insulin in two-site enzyme immunoassay.

Advanced ImmunoChemical, Inc. ANTI-CA-125 MAB

MONOCLONAL ANTIBODIES/Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with CA125 antigen purified from human ovarian carcinoma (MW > 1 MDa).Specificity:CA125 antigen.MAbs X52cc, X75, X325: epitope specificity group B (ISOBM classification) similar to M-11.MAb X306cc: epitope specificity group A (ISOBM classification) similar to OC125.MAb Isotypes:IgG1 for all MAbsApplications:Detection of CA125 antigen. Suggested pairs for sandwich immunoassay (capture-detection):X306cc – X52ccX306cc – X325X306cc – X75All MAbs are working in Western blotting.paraffin embedded tissue.Purification:Ion exchange chromatographyPresentation:PBS, pH 7.4, 0.09 % sodium azide (NaN3)0.15 M NaCl, 10 mM Tris, pH 7.5, 0.05% sodium azide (NaN3) for X75, X325Storage Conditions:+ 4 °C (+2°C…+8°C allowed).

Advanced ImmunoChemical, Inc. ANTI-CANINE NT-PROBNP MAB

MONOCLONAL ANTIBODIES/Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with: recombinant canine NT-proBNP with tag expressed in E. coli (CaNT90) recombinant canine NT-proBNP expressed in E. coli as a fusion protein with carrier (CaNT89, CaNT19, CaNT46, CaNT925) synthetic peptides corresponding to 3-26 a.a.r., 13-33 a.a.r., and 64- 80 a.a.r. (CaNT49, CaNT53) of canine NT-proBNPSpecificity:Canine NT-proBNP and proBNP.MAb Isotypes:IgG1 for MAbs CaNT89, CaNT90, CaNT19, CaNT46, CaNT49, CaNT53Applications:Quantification of canine NT-proBNP in plasma of dogs. Recommended pairs for sandwich immunoassay (capture-detection):CaNT90 – CaNT89CaNT19 – CaNT89CaNT90 – CaNT53.

Advanced ImmunoChemical, Inc. ANTI-HUMAN SAA MAB

MONOCLONAL ANTIBODIES/MAbs:SAA6, VSA6 ,VSA25Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cellsof Balb/c mice immunized with human recombinant SAA, conjugated with carrier protein.Specificity:Human recombinant SAAMAb Isotypes:IgG1 for MAbs SAA6, VSA6, VSA25Applications:SAA immunoassay. All MAbs recognize SAA in ELISA and Western blotting.Recommended pairs for sandwich immunoassay (capture-detection):VSA25 – VSA31VSA6 – VSA38Purification:Chromatography on protein A SepharosePresentation:PBS, pH 7.4, 0.1 % sodium azide (NaN3)Storage Conditions:+ 4 °C

Advanced ImmunoChemical, Inc. PROCALCITONIN, HU RECOMBINANT

ANTIGENS/Source:E. coli expression systemDescription:Consists of a polypeptide corresponding to 1-116 amino acid residues of human procalcitonin. Does not contain any tags.Applications:An antigen for immunodetection of human PCTAnalysis:Purity > 95 %Immunoreactivity of recombinant human PCT is checked in sandwich immunoassay with specific monoclonal antibodies.Purification:Affinity and reverse-phase chromatographyPresentation:Lyophilized from 20 mM Tris-HCl, pH 8.0, 150 mM NaCl.Product is recommended to reconstitute with deionized water.Storage Conditions:Before and after reconstitution at -20 °C (between -15 and -30 °C allowed)

Advanced ImmunoChemical, Inc. Human Papillomavirus L1 Protein (HPVL1), Recombinant, Type 18, Analysis:Purity > 90 %

This item is manufactured by HyTest, Ltd and distributed by Advanced ImmunoChemical, Inc. Antigens/ Infectious Diseases / Human Papillomavirus L1 protein (HPVL1), recombinant, type 18. Analysis:Purity > 90 % (SDS-PAGE).Each lot is tested for protein content (Lowry method), lipid content (Total lipid), purity (SDS-PAGE), carbohydrate content (Anthrone method), antigen content (Immunoblot).Immunological activity:Tested in quantitative immunoblot with anti-HPVL1 antibodies.Purification:Purification is conducted from broken yeast cells by following scheme: clarification, ammonium sulfate precipitation, ultrafiltration, cation exchange chromatography, zonal centrifugation, size-exclusion chromatography, sterile filtration.Presentation:Presentation: 20 mM KH2PO4 (pH 7.2), 500 mM NaCl, 1.7 mM EDTA, 0.01% Tween-80, 1 mM 2-MercaptoethanoStorage Conditions:+ 4 °C (between +2 and +8 °C allowed)Material safety note:This product is sold for research use only.