General Western Blot Reagents
Abcam 20X Wash Buffer.
20X Wash Buffer.
The product is subject to the following: Abcam Restricted Use Statement
Abcam Glucose Uptake Assay Kit (Fluorometric)
Glucose Uptake Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Abcam Green CMFDA (5-Chloromethylfluorescein diacetate), Green fluorescent dye, 1MG
MW 464.8 Da, Purity >95%. Cell-permeable green fluorescent dye. Does not affect cell viability or proliferation. Transforms intracellularly into a cell-impermeant, fluorescent product. Displays fluorescence for at least 72 hours. Brightly fluorescent, non-toxic and stable at physiological pH. Allows for multigenerational tracking of cellular movements.
The product is subject to the following: Abcam Restricted Use Statement
Abcam Mertansine (DM1 Compound), microtubule-targeted cytoto x ic agent, 10MG
MW 738.3 Da. Potent microtubule-targeted cytotoxic agent. Induces mitotic arrest. Inhibits microtubule polymerization. Shows antitumor effects in vivo. .
The product is subject to the following: Abcam Restricted Use Statement
Abcam x TT sodium salt, Tetrazolium salt, 100MG
MW 673.53 g/mol. Tetrazolium sodium salt for use in colorimetric assays for cell viability and proliferation. Cleavage of XTT by dehydrogenase enzymes of metabolically active cells yields a highly colored, water soluble formazan product, an advantage over the use of MTT due to MTT requiring a formazan crystal solubilization step. Has been used to asses valvular injury for testing cryopreserved cardiac valves.
The product is subject to the following: Abcam Restricted Use Statement
Sigma Aldrich Fine Chemicals Biosciences HiTrapR IMAC Fast Flow Cyt
HiTrap(R) IMAC FF is prepacked with IMAC Sepharose(R) 6 Fast Flow. The medium is charged with the metalion of your choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.
Rockland Immunochemicals Mouse TrueBlot Set (with IP Agarose beads) - 88-7788-31
Mouse IgG TrueBlot ULTRA is provided as 1000X solution. We recommend incubating the blots from minigels in sealed bags with minimal volume (2-3 mLs). If used conservatively at 2.5mls/blot will yield enough reagent for 20 blots. Note that there are three key procedural considerations: 1. Protein A or G beads may be used with the mouse, goat and sheep TrueBlot secondaries, but not with the rabbit TrueBlot secondary. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot. We recommend using a large bore pipet to pipet up the liquid for use. To achieve best results when detecting mouse IgG1 subtypes, we recommend performing a dot blot or titration to determine the optimal dilution. Specific conditions for each protein and antibody combination should be specifically optimized by the end user.
Genhunter Corporation PerfectWestern, 3-Sectional-Strip, Black
PerfectWestern, 3-Sectional-Strip, Black (Qty. 1-4)
Abcam 1X Base Buffer.
1X Base Buffer.
The product is subject to the following: Abcam Restricted Use Statement
ProteinSimple Fluorescent 5x Master Mix 1
Compatible with Jess, Abby, Wes, Peggy Sue and Sally Sue systems. Pack includes 8 packets with one each of the Single Use FL Standard (29 kDa system control protein) and Single Use DTT vials. Compatible with 12-230 Separation Modules.
Abcam Acetylcholinesterase Assay Kit (Fluorometric Green)
Acetylcholinesterase Assay Kit (Fluorometric Green)
The product is subject to the following: Abcam Restricted Use Statement
Abcam Hydrogen Peroxide Assay Kit (Fluorometric Near Infrared)
Hydrogen Peroxide Assay Kit (Fluorometric Near Infrared)
The product is subject to the following: Abcam Restricted Use Statement
Abcam E2 Ubiquitin Conjugation Kit
E2 Ubiquitin Conjugation Kit
The product is subject to the following: Abcam Restricted Use Statement
Abcam Hydrogen Peroxide Assay Kit (Cell based)
Hydrogen Peroxide Assay Kit (Cell based)
The product is subject to the following: Abcam Restricted Use Statement