Enzymes and Inhibitors
T4 RNA ligase II, 750 U, G-Biosciences
T4 RNA Ligase II (gp24.1) repairs nicks in dsRNA and can also be used to ligate the 3' terminal OH of RNA to the 5'-terminal phosphate of DNA in a double-stranded structure.
Thermolabile UDG, 500 U, G-Biosciences
Thermolabile Uracil-DNA Glycosylase (UDG), originating from the species Gadus morhua, removes uracil from DNA by hydrolyzing the N-glycosylic bond between the deoxyribose and the base leaving an AP (apurinic or apyrimidinic) site.
Alkaline Phosphatase, 2500 U, G-Biosciences
TAB5 Alkaline Phosphatase is a heat labile alkaline phosphatase. It catalyzes the dephosphorylation of phosphomonoesters at both 5' and 3' ends of DNA and RNA molecules in a nonspecific manner. Additionally, it eliminates phosphate groups from the 5' and 3' positions of DNA, RNA, and dNTPs.
Klenow Exo, 1000 U, G-Biosciences
Klenow fragment (Exo-) is a mesophilic DNA polymerase deficient in both proofreading (3′→5′) and nick-translation (5′→3′) nuclease activities, with a moderate strand displacement activity during DNA synthesis.
E. coli Pyrophosphatase (PPase), 50 U, G-Biosciences
E. coli Inorganic Pyrophosphatase (PPase) speeds up the breakdown of inorganic pyrophosphate (PPi) into orthophosphate.
E. coli Gyrase, 100 U, G-Biosciences
Gyrase is a type IA DNA topoisomerase and it is involved in bacterial DNA supercoiling.
E. coli Exonuclease I, 5000 U, G-Biosciences
Exonuclease I (Exo I), is a highly processive single-stranded DNA-specific exonuclease which removes mono- and dinucleotides from the 3' end of single-stranded DNA molecules (3' to 5' exonuclease activity) leaving double-stranded molecules and the 5' end of single stranded molecules intact.
G-Biosciences RNase Inhibitor (Murine)
RNase Inhibitor (Murine) is used for first strand cDNA synthesis, RT-qPCR.
G-Biosciences T5 Exonuclease
T5 Exonuclease is a highly efficient 5'-to-3' exonuclease for either ssDNA or dsDNA. It also has ssDNA specific endonuclease activity in the presence of magnesium ions.
G-Biosciences T4 Polynucleotide Kinase (PNK)
T4 Polynucleotide Kinase (PNK) exhibits 5' polynucleotide kinase and 3' phosphatase activity.
G-Biosciences Taq DNA Ligase
Taq DNA Ligase uses NAD+ as a cofactor to catalyze the formation of a phosphodiester bond in duplex DNA containing adjacent 5'-phosphoryl and 3'-hydroxyl termini.
T4 RNA ligase I, 5000 U, G-Biosciences
T4 RNA Ligase I (gp63) catalyzes the ATP-dependent ligation of single-stranded nucleic acids by joining a 5′ phosphate to a 3′ hydroxyl. It is most efficient at ligating the 3′-OH of RNA to the 5′-phosphate of RNA or DNA in single-stranded contexts.
T7 DNA Ligase, 75000 U, G-Biosciences
T7 DNA Ligase is an ATP-dependent enzyme from bacteriophage T7 that catalyzes phosphodiester bond formation between 5′ phosphate and 3′ hydroxyl termini of duplex DNA.