Antibody Panels and Kits

196 – 210 of 920 results

196

MilliporeSigma™ Upstate™ TCF-4α, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Mouse Monoclonal Antibody

Pricing & Availability
Specifications

Content And Storage	-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Host Species	Mouse
Applications	ChIP Assay,Dot Blot,Western Blot
Form	Purified
Gene Accession No.	Q9NQB0
Isotype	IgG2a
Includes	This ChIPAb+ TCF-4 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Antigen	TCF-4α
Regulatory Status	RUO
Gene Symbols	TCF4, TCF-4, TCF7L2
Purification Method	Protein G chromatography
Gene ID (Entrez)	CAB97213.1
Formulation	Anti-TCF-4 (mouse monoclonal). One vial containing 50μg of purified mouse monoclonal IgG2a in 50μL of buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol. Normal Mouse IgG. Two vials containing 25μg of purified IgG in 25μL of storage buffer containing 0.1% sodium azide. ChIP Primers, SP5 promote. One vial containing 75μL of 5μM of each primer specific for human SP5 promotor. FOR: GGG TCT CCA GGC GGC AAG; REV: AGC GAA AGC AAA TCC TTT GAA TCC
Immunogen	Amino acids 31-331 of human TCF-4.
Classification	Monoclonal
Primary or Secondary	Primary
Test Specificity	This antibody recognizes TCF-4

197

MilliporeSigma™ Upstate™ Dimethyl-Histone H3 (Lys36), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Rabbit Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Host Species	Rabbit
Applications	ChIP Assay,Electromobility Shift Assay,Immunoprecipitation,Western Blot
Form	Serum
Gene Accession No.	Q16695
Includes	This ChIPAb+ Validated Antibody and Primer Set conveniently includes the antibody, matched IgG negative control antibody and set control PCR primers that detect a known positive locus.
Antigen	Dimethyl-Histone H3 (Lys36)
Regulatory Status	RUO
Gene Symbols	H3.4, H3/g , H3/t, H3FT, H3T, H3t, MGC126886, MGC126888, OTTHUMP00000037945
Gene ID (Entrez)	NP_003484
Formulation	Anti-Dimethyl Histone H3 (Lys36) (Rabbit Polyclonal). One vial containing 25μL rabbit polyclonal serum containing 0.05% sodium azide, before the addition of 30% glycerol. Normal Rabbit Serum. One vial containing 25μL antiserum containing 0.05% sodium azide. ChIP Primers, GAPDH coding D2. One vial containing 75μL of 5μM of each primer specific for human GAPDH coding region. (chr12:6647453+6647539, hg 19 build). FOR: 5’ GCC ATG TAG ACC CCT TGA AGA GC 3’; REV: 5’ ACT GGT TGA GCA CAG GGT ACT TTA T 3’
Immunogen	KLH-conjugated linear peptide corresponding to a region near the N-terminus of human Histone H3 dimethylated at Lys36.
Classification	Polyclonal
Primary or Secondary	Primary
Test Specificity	Recognizes Dimethyl Histone H3 (Lys36). No detectable cross-reactivity unmethylated, mono, or dimethylated Lys36. No detectable cross reactivity to other histone modifications as measured by AbSurance Histone Antibody Specificity Arrays.

198

MilliporeSigma™ Upstate™ CBX8 Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Rabbit Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Host Species	Rabbit
Applications	ChIP Assay,Western Blot
Form	Purified
Gene Accession No.	Q9HC52
Includes	Use ChIPAb+ CBX8 ChIP Validated rabbit polyclonal antibody for the detection of CBX8 also known as Chromobox protein homolog 8 and Polycomb 3 homolog.
Antigen	CBX8
Regulatory Status	RUO
Gene Symbols	HPC3, PC3, Pc3, RC1, hPc3
Purification Method	Affinity Purified
Gene ID (Entrez)	NP_065700
Formulation	Anti-CBX8 (Rabbit Polyclonal). One vial containing 50μg affinity purified rabbit polyclonal in 72μL buffer containing Tris-citrate/phosphate buffer (pH 7 to 8) and 0.09% sodium azide, before the addition of glycerol to 30% (0.7mg/mL final). Normal Rabbit IgG. One vial containing 125μg of purified Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. ChIP Primers, HOXA2 Upstream. One vial containing 75μL of 5μM of each primer specific for human HOXA2 promoter region (chr7:27142948-27143085, hg19 build). FOR: AGG AAA GAT TTT GGT TGG GAA G; REV: AAA AAG AGG GAA AGG GAC AGA C
Classification	Polyclonal
Primary or Secondary	Primary

199

MilliporeSigma™ Upstate™ ChIPAb+™ Dimethyl-Histone H3 (Lys79)α, Rabbit Monoclonal, ChIP Validated Antibody and Primer Set

Rabbit Monoclonal Antibody

Pricing & Availability
Specifications

Content And Storage	-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Host Species	Rabbit
Applications	ChIP Assay,Western Blot
Form	Culture Supernatant
Gene Accession No.	Q16695
Isotype	IgG
Includes	This ChIPAb+ Dimethyl-Histone H3 (Lys79) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Antigen	ChIPAb+™ Dimethyl-Histone H3 (Lys79)α
Regulatory Status	RUO
Gene Symbols	H3F3A, H3F3B
Gene ID (Entrez)	NM_003493
Formulation	Anti-Dimethyl-Histone H3 (Lys79) (rabbit Monoclonal), One vial containing 100μL of cultured supernatant in 0.05% sodium azide. Negative Control Supernatant, One vial containing 100μL of cultured supernatant in 0.05% sodium azide. Control Primers. One vial containing 75μL of 5μM of each primer specific for human GAPDH. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA
Immunogen	KLH-conjugated synthetic peptide containing the sequence DF_me2KTD corresponding to dimethyl-lysine at position 79 of histone H3. The immunizing sequence is identical in human,mouse,rat and yeast. A C-terminal cysteine was added to facilitate conjugation.
Classification	Monoclonal
Primary or Secondary	Primary
Test Specificity	Recognizes dimethyl-histone H3 (Lys79).

200

MilliporeSigma™ Upstate™ ChIPAb+™ Phospho-Histone H3 (Thr3)α, Rabbit Monoclonal, ChIP Validated Antibody and Primer Set

Rabbit Monoclonal Antibody

Pricing & Availability
Specifications

Content And Storage	-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Host Species	Rabbit
Applications	ChIP Assay,Dot Blot,Western Blot
Form	Culture Supernatant
Gene Accession No.	Q16695
Includes	This ChIPAb+ Phospho-Histone H3 (Thr3) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Antigen	ChIPAb+™ Phospho-Histone H3 (Thr3)α
Regulatory Status	RUO
Gene Symbols	HIST3H3, H3FT, MGC126886, H3t, MGC126888, H3.4, H3T, H3/g, H3/t
Gene ID (Entrez)	NM_003493
Formulation	Anti-Phospho-Histone H3 (Thr3) (rabbit monoclonal). One vial containing 50μL of cultured supernantant in 0.05% sodium azide. Negative Control Supernatant. One vial containing 100μL of cultured supernatant in 0.05% sodium azide. Control Primers, human GAPDH promoter. One vial containing 75μL of 5μM of each primer specific for human GAPDH. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA
Immunogen	Peptide containing the sequence [RT_trimKQ] in which lysine 4 is trimethylated on human histone H3
Classification	Monoclonal
Primary or Secondary	Primary
Test Specificity	Histone H3 phosphorylated at Threonine 3; cross-reactivity with phospho-histone H3 Thr22 detected in some applications

201

MilliporeSigma™ Upstate™ Trimethyl-Histone H3 (Lys9)α, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Mouse Monoclonal Antibody

Pricing & Availability
Specifications

Content And Storage	2°C to 8°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at -20°C; Avoid repeated freeze/thaw cycles
Host Species	Mouse
Applications	ChIP Assay,Western Blot
Form	Purified
Gene Accession No.	Q16695
Isotype	IgG1 κ
Includes	This ChIPAb+ Trimethyl-Histone H3 (Lys9) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Antigen	Trimethyl-Histone H3 (Lys9)α
Regulatory Status	RUO
Gene Symbols	H3., H3/g, H3/t, H3FT, H3T, H3t, MGC126886, MGC126888, OTTHUMP00000037945
Purification Method	Protein G purified
Gene ID (Entrez)	NM_003493
Formulation	Anti-Trimethyl-Histone H3 (Lys9) (mouse monoclonal). One vial containing 25μL of purified mouse monoclonal IgG1 in PBS with 0.05% sodium azide before the addition of glycerol to 30%. Normal Mouse IgG. One vial containing 25μg of purified IgG in 25μL of storage buffer containing 0.1% sodium azide. Control Primers, ZNF554. One vial containing 75μL of 5μM of each primer specific for the 3’ end of human ZNF554. FOR: CGG GGA AAA GCC CTA TAA AT; REV: TCC ACA TTC ACT GCA TTC GT
Immunogen	KLH-conjugated synthetic peptide corresponding to human Histone H3 trimethylated on Lys9.
Classification	Monoclonal
Primary or Secondary	Primary
Test Specificity	This antibody recognizes Histone H3 trimethylated at Lys9. Some cross-reactivity with dimethyl Lys9 is detected at higher concentrations, but the difference in specificity is >27-fold1. Phosphorylation of Ser10 interferes with antibody binding to trimethyl Lys91.

202

MilliporeSigma™ Upstate™ SMRTα, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Mouse Monoclonal Antibody

Pricing & Availability
Specifications

Content And Storage	-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Host Species	Mouse
Applications	ChIP Assay,Electromobility Shift Assay,Immunocytochemistry,Immunohistochemistry,Immunoprecipitation,Western Blot
Form	Ascites
Gene Accession No.	Q9Y618
Isotype	IgG2a κ
Includes	The ChIPAb+ SMRT set includes the SMRT antibody, a negative control mouse ascites and qPCR primers which amplify a 299bp region of human IκBα promoter.
Antigen	SMRTα
Regulatory Status	RUO
Gene Symbols	NCOR2, CTG26, N-CoR2, SMAP270, SMRT, SMRTE, SMRTE-tau, TNRC14, TRAC, TRAC-1, TRAC1
Purification Method	Unpurified
Gene ID (Entrez)	NP_001070729.1
Formulation	Anti-SMRT (mouse monoclonal). One vial containing 50μL of unpurified mouse monoclonal ascites with 0.05% sodium azide. Negative Ascites (mouse). One vial containing 50μL of mouse ascites with 0.05% sodium azide. ChIP Primers, IκBα promoter. One vial containing 75μL of 5μM of each primer specific for human IĸBα promoter. FOR: GAC GAC CCC AAT TCA AAT CG; REV: TCA GGC TCG GGG AAT TTC C
Immunogen	Recombinant protein corresponding to human SMRT.
Classification	Monoclonal
Primary or Secondary	Primary
Test Specificity	Recognizes SMRT.

203

MilliporeSigma™ Upstate™ Acetyl-Histone H4 (Lys5)α, Rabbit Monoclonal, ChIP Validated Antibody and Primer Set

Rabbit Monoclonal Antibody

Pricing & Availability
Specifications

Content And Storage	-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Host Species	Rabbit
Applications	ChIP Assay,Immunoprecipitation,Western Blot
Form	Culture Supernatant
Gene Accession No.	P62805
Isotype	IgG
Includes	This ChIPAb+ Acetyl-Histone H4 (Lys5) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Antigen	Acetyl-Histone H4 (Lys5)α
Regulatory Status	RUO
Gene Symbols	HIST1H4A, H4/J, HIST1H4F, HIST1H4L, H4FN, H4FA, H4FH, HIST1H4H, H4F2, H4FM, H4/A, H4FK, H4FG, HIST2H4, H4FB, H4/K, HIST1H4I, H4/N, HIST1H4B, H4FE, H4/M, H4/a, HIST1H4E, HIST4H4, HIST2H4A, H4FC, H4FI, H4/H, HIST1H4C, H4/G, HIST1H4K, H4FJ, H4FD, H4/I, H4/B, H4/D, H4/C, HIST1H4J, HIST1H4D, H4/E
Gene ID (Entrez)	NP_001029249
Formulation	Anti-Acetyl-Histone H4 (Lys5) (rabbit monoclonal IgG). One vial containing 50μL of rabbit monoclonal IgG cell culture supernatant in 0.1% sodium azide. Negative Control Supernatant (rabbit). One vial containing 50μL of cultured supernatant in 0.05% sodium azide. Control Primers. One vial containing 75μL of 5μM each primer specific for the human GAPDH promoter. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA
Immunogen	Peptide corresponding to Histone H4 containing the sequence [GRG-AcK-GGK] on which Lys5 is acetylated.
Classification	Monoclonal
Primary or Secondary	Primary
Test Specificity	Recognizes Histone H4 acetylated on Lys5.

204

MilliporeSigma™ Upstate™ Monomethyl-Histone H3 (Lys9)α, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Mouse Monoclonal Antibody

Pricing & Availability
Specifications

Content And Storage	−20°C for up to 12 months; Avoid repeated freeze/thaw cycles
Host Species	Mouse
Applications	ChIP Assay,ChIP sequencing (ChIP-seq),Western Blot
Form	Purified
Gene Accession No.	Q66I33
Isotype	IgG
Includes	This ChIPAb+ Validated Antibody and Primer Set conveniently includes the antibody, matched IgG negative control antibody and set control PCR primers that detect a known positive locus.
Antigen	Monomethyl-Histone H3 (Lys9)α
Regulatory Status	RUO
Purification Method	Protein G purified
Gene ID (Entrez)	NM_003493
Formulation	Anti-monomethyl-Histone H3 (Lys9) (mouse monoclonal IgG2aқ, clone CMA306). One vial containing 50μg of protein G purified antibody in 50μL PBS containing 0.05% sodium azide. Normal Mouse IgG. Two vials containing 25μg purified Mouse IgG in 25μL storage buffer containing 0.1% sodium azide. ChIP Primers GAPDH Coding region. One vial containing 75μL of 5μM of each primer specific for a region of the human GAPDH coding region. FOR: GGC TCC CAC CTT TCT CAT CC; REV: GGC CAT CCA CAG TCT TCT GG
Immunogen	The monomethyl-histone H3 (Lys9) purified antibody is made against a synthetic peptide (monomethylated at Lys9) corresponding to amino acids 1-18 of Histone H3.
Classification	Monoclonal
Primary or Secondary	Primary
Test Specificity	Recognizes histone H3, Mr 17kDa, monomethylated at Lys9.

205

MilliporeSigma™ Upstate™ SUZ12α, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Mouse Monoclonal Antibody

Pricing & Availability
Specifications

Content And Storage	-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Host Species	Mouse
Applications	ChIP Assay,Dot Blot,Western Blot
Form	Purified
Gene Accession No.	Q15022
Isotype	IgG1 κ
Includes	This ChIPAb+ SUZ12 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Antigen	SUZ12α
Regulatory Status	RUO
Gene Symbols	SUZ12; JJAZ1; ChET9
Gene ID (Entrez)	NP_056170
Formulation	Anti-SUZ12 (mouse monoclonal IgG). One vial containing 50μg of protein A purified antibody in PBS containing 0.05% sodium azide. May contain 30% glycerol (see certificate of analysis). Normal Mouse IgG. Two vials containing 25μg of purified mouse IgG in 25μL of storage buffer containing 0.1% sodium azide. ChIP Primers, HoxA2 upstream. One vial containing 75μL of 5μM of each primer specific for the promoter region of human HoxA2. FOR: AGG AAA GAT TTT GGT TGG GAA G; REV: AAA AAG AGG GAA AGG GAC AGA C
Immunogen	SUZ12,purified antibody is made against human SUZ12.
Classification	Monoclonal
Primary or Secondary	Primary
Test Specificity	Recognizes SUZ12, Mr 95kDa.

206

MilliporeSigma™ Upstate™ Trimethyl-Histone H3 (Lys4)α, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Mouse Monoclonal Antibody

Pricing & Availability
Specifications

Content And Storage	−20°C for up to 12 months; Avoid repeated freeze/thaw cycles
Host Species	Mouse
Applications	ChIP Assay,Immunocytochemistry,Western Blot
Form	Purified
Gene Accession No.	Q66I33
Isotype	IgG1 κ
Includes	This ChIPAb+ Trimethyl-Histone H3 (Lys4) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Antigen	Trimethyl-Histone H3 (Lys4)α
Regulatory Status	RUO
Purification Method	Protein G purified
Gene ID (Entrez)	NM_003493
Formulation	Anti-trimethyl-Histone H3 (Lys4) (mouse monoclonal IgG1, Clone CMA304). One vial containing 50μg of protein G purified antibody in 50μL PBS containing 0.05% sodium. Normal Mouse IgG. Two vials containing 25μg purified mouse IgG in 25μL storage buffer containing 0.1% sodium azide. Control Primers. One vial containing 75μL of 5μM of each primer specific for the promoter region of GAPDH. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA
Immunogen	The trimethyl-histone H3 (Lys4) purified antibody is made against a synthetic peptide (trimethylated at Lys4) corresponding to amino acids 1-12 of human histone H3.
Classification	Monoclonal
Primary or Secondary	Primary
Test Specificity	Recognizes histone H3, Mr 17kDa, trimethylated at Lys4.

207

MilliporeSigma™ Upstate™ Dimethyl-Histone H3 (Lys4)α, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Mouse Monoclonal Antibody

Pricing & Availability
Specifications

Content And Storage	−20°C for up to 12 months; Avoid repeated freeze/thaw cycles
Host Species	Mouse
Applications	Multiplex,ChIP sequencing (ChIP-seq),Dot Blot,Immunoassay,Western Blot
Form	Purified
Gene Accession No.	Q66I33
Isotype	IgG2b κ
Includes	This ChIPAb+ Dimethyl-Histone H3 (Lys4) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Antigen	Dimethyl-Histone H3 (Lys4)α
Regulatory Status	RUO
Gene ID (Entrez)	NM_003493
Formulation	Anti-dimethyl-Histone H3 (Lys4) (mouse monoclonal IgG1, clone CMA303). One vial containing 50μg of protein G purified antibody in 50μL PBS containing 0.05% sodium azide. Normal Mouse IgG. Two vials containing 25μg purified Mouse IgG in 25μL storage buffer containing 0.1% sodium azide. ChIP Primers GAPDH Coding. One vial containing 75μL of 5μM of each primer specific for the coding region human GAPDH. FOR: GGC TCC CAC CTT TCT CAT CC; REV: GGC CAT CCA CAG TCT TCT GG
Immunogen	The dimethyl-histone H3 (Lys4) purified antibody is made against a synthetic peptide (dimethylated at Lys4) corresponding to amino acids 1-12 of histone H3.
Classification	Monoclonal
Primary or Secondary	Primary
Test Specificity	Recognizes histone H3, Mr 17kDa, dimethylated at lysine 4.

208

MilliporeSigma™ Upstate™ Trimethyl-Histone H3 (Lys4)α, Rabbit Monoclonal, ChIP Validated Antibody and Primer Set

Rabbit Monoclonal Antibody

Pricing & Availability
Specifications

Content And Storage	−20°C for one year from date of shipment
Host Species	Rabbit
Applications	ChIP Assay,ChIP sequencing (ChIP-seq),Western Blot
Form	Purified
Gene Accession No.	Q66I33
Isotype	IgG
Includes	Trimethyl-Histone H3 (Lys4) ChIP validated Antibody and Primer set including the ChIP-grade antibody and the specific control PCR primers used for chromatin immunoprecipitation of H3K4Me3.
Antigen	Trimethyl-Histone H3 (Lys4)α
Regulatory Status	RUO
Gene Symbols	H3F3A, H3F3B, H3F3
Gene ID (Entrez)	NM_003493
Formulation	Anti-Trimethyl-Histone H3 (Lys4) recombin-ant rabbit monoclonal IgG. One vial containing 75μL of protein A purified rabbit IgG in storage buffer (0.1M Tris-Glycine pH 7.4, 0.15M NaCl, 0.05% NaN3, with the addition of 40% glycerol. Normal Rabbit IgG. One vial containing 75μL of normal rabbit IgG. Control Primers. One vial containing 75μL of 5μM of each primer specific for human GAPDH. FOR: TAC TAG CGG TTT TAC GGG CG REV: TCG AAC AGG AGG AGC AGA GAG CGA
Immunogen	The trimethyl-histone H3 (Lys4) antibody is made against a BSA-conjugated,synthetic peptide containing the sequence …RT[me3K]QT… in which me3K corresponds to trimethyl-lysine 4 of human histone H3
Classification	Monoclonal
Primary or Secondary	Primary
Test Specificity	Trimethyl-Histone H3 (Lys4)

209

MilliporeSigma™ Upstate™ Trimethyl-Histone H3 (Lys27), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Rabbit Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	−20°C for one year
Host Species	Rabbit
Applications	ChIP Assay,Western Blot
Form	Purified
Gene Accession No.	Q16695
Includes	Trimethyl-Histone H3 (Lys27) ChIP validated Antibody and Primer set including the ChIP-grade antibody and the specific control PCR primers used for chromatin immunoprecipitation of H3K27Me3.
Antigen	Trimethyl-Histone H3 (Lys27)
Regulatory Status	RUO
Gene Symbols	H3F3A, MGC87783, H3.3A, MGC87782, H3F3, H3.3B, H3F3B
Gene ID (Entrez)	NP_003484
Formulation	Anti-trimethyl-Histone H3 (Lys27) (rabbit polyclonal IgG). One vial containing 100μg protein A purified IgG in 100μL buffer containing 0.1 M Tris-Glycine, pH 7.4, 0.15M NaCl with 0.05% sodium azide. Normal Rabbit IgG. One vial containing 125μg purified Rabbit IgG in 125μL storage buffer containing 0.1% sodium azide. ChIP Primers human alpha-Satellite. One vial containing 75μL of 5μM of each control primer specific for human alpha-Satellite. FOR: CTG CAC TAC CTG AAG AGG AC; REV: GAT GGT TCA ACA CTC TTA CA
Immunogen	KLH-conjugated,synthetic 2X-branched peptide containing the sequence ...AR(me3K)SAP... in which me3K corresponds to trimethyl-lysine at residue 27 of human Histone H3.
Classification	Polyclonal
Primary or Secondary	Primary
Test Specificity	trimethyl-Histone H3 (Lys27)

210

MilliporeSigma™ Upstate™ LEF1α, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Mouse Monoclonal Antibody

Pricing & Availability
Specifications

Content And Storage	−20°C for one year from date of shipment
Host Species	Mouse
Applications	ChIP Assay,Western Blot
Form	Purified
Gene Accession No.	Q9UJU2
Isotype	IgG1
Includes	This ChIPAb+ LEF1 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Antigen	LEF1α
Regulatory Status	RUO
Gene Symbols	LEF1, DKFZp586H0919, TCF1ALPHA, LEF-1, TCF1-alpha
Gene ID (Entrez)	NM_016269.2
Formulation	Anti-LEF1 (mouse IgG1). 1 vial containing 100mg of thiophilic and size exclusion chromatography purified mouse IgG1 in 100mL of phosphate buffered saline with sodium azide. The antibody is made against amino acid residues 1-85 of human LEF1 and can recognize human and mouse LEF1. It does not cross-react with TCF-3 or TCF-4. Normal Mouse IgG. One vial containing 125μg of normal mouse IgG in 125μL volume. ChIP primers c-myc. One vial containing 75mL of 5μM of each control primer specific for human c-myc promoter. FOR: CCC AAA AAA AGG CAC GGA A; REV: TAT TGG AAA TGC GGT CAT GC
Immunogen	The antibody is made against amino acid residues 1-85 of human LEF1.
Classification	Monoclonal
Primary or Secondary	Primary
Test Specificity	Recognizes LEF1 (lymphoid enhancer-binding factor 1).

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