Restriction Enzymes
New England Biolabs, Inc. ScaI-HF – 1000 units
A high fidelity restriction endonuclease that recognizes the sequence AGT^ACT. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
New England Biolabs, Inc. SphI-HF – 500 units
A high fidelity restriction endonuclease that recognizes the sequence GCATG^C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
New England Biolabs, Inc. Hpy166II – 1000 units
A restriction endonuclease that recognizes the sequence GTN^_NAC.
New England Biolabs, Inc. Nb.BtsI – 1000 units
A restriction endonuclease that recognizes the sequence GCAGTG_NN.
New England Biolabs, Inc. FatI – 50 units
A restriction endonuclease that recognizes the sequence ^CATG_.
New England Biolabs, Inc. Nb.BsrDI – 1000 units
A restriction endonuclease that recognizes the sequence GCAATG_NN.
New England Biolabs, Inc. Nb.BsmI – 1000 units
A restriction endonuclease that recognizes the sequence GAATG_CN.
New England Biolabs, Inc. CviQI – 2000 units
A restriction endonuclease that recognizes the sequence G^TA_C.
New England Biolabs, Inc. PleI – 1000 units
A restriction endonuclease that recognizes the sequence GAGTCNNNN^N_.
New England Biolabs, Inc. BsaJI – 1000 units
A restriction endonuclease that recognizes the sequence C^CNNG_G.
Supply Solutions Sigma Aldrich - Phosphatase,alkaline(AP),MB Grade(1U/l) * Third Party Item
Sigma Aldrich - Phosphatase,alkaline(AP),MB Grade(1U/l) * Third Party Item
New England Biolabs, Inc. BsaXI – 500 units
A restriction endonuclease that recognizes the sequence _NNN^NNNNNNNNNACNNNNNCTCCNNNNNNN_NNN^.
Sorbent Technologies Inc Silica for DCC, w/UV254, 60A, 63-200um, 500g
Silica for Dry Column Chromatography (DCC) is a fast, easy, and efficient method for separating and/or purifying industrial quantities of compounds. DCC links analytical thin layer chromatography (TLC) with preparative column chromatography (CC). Like TLC, DCC is a "non-elution" method utilizing Nylon Foil Tubing as a support. In TLC solvent flow is by capillary action going against gravity whereas in DCC, solvent flow is by gravity. Like Column Chromatography, columns of most any size may be filled in DCC to separate or clean up very large quantities of materials. Silica Gel for DCC consists of a UV254 fluorescent indicator and a silica gel with a porosity of 60A, particle distribution of 63-200um, and a quantity of 500g.
INTACT GENOMICS INC PEmax Enzyme 80 µg
Intact Genomics (IG) PEmax enzyme is the purified recombinant Streptococcus pyogenes Cas9 nickase mutant (H840A)-Murine Moloney Leukemia Virus-Reverse Transcriptase (MMLV-RT) fusion protein3-4 containing a nuclear localization signal (NLS) at the C-terminal for targeting to the nucleus. This enzyme is designed to perform CRISPR prime-editing3-4. The physical purity of this enzyme is ≥85% as assessed by SDS-PAGE with Coomassie blue staining.Product Includes: PEmax enzyme 5x PEmax Nicking Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM MgCl2, 0.1 mM EDTA, pH 6.5 @ 25 °CStore all components at -20°C.