Cell Culture Reagents, Antibiotics and Supplements
Invivogen Puromycin, Selective antibiotic for the Pac gene, 58-58-2, 100mg (10x1ml)
Formula: C22H29N7O5 • 2HCl, Purity: ≥ 98% (HPLC), Molecular weight: 544.3, Endotoxin level: < 5 EU/mg, Puromycin is an aminonucleoside antibiotic produced by Streptomyces alboniger. It specifically inhibits peptidyl transfer on both prokaryotic and eukaryotic ribosomes. This antibiotic inhibits the growth of Gram positive bacteria and various animal and insect cells.
Lonza Walkersville Trypsin Neutralizing Solution, 100 mL
Trypsin Neutralizing Solution (TNS) is used to effectively neutralize Trypsin/EDTA. Lonza's TNS is availableindividually or as a component in our ReagentPack Subculture Reagents (CC-5034) which includes Trypsin and HEPES buffer.Content:1 x Bottle Trypsin Neutralizing Solution, 100 mL
Sigma Aldrich Fine Chemicals Biosciences ProClin™ 300. Pkg of 50mL
For use in biological diagnostic assays and IVDs only.
Invivogen Selective antibiotic for the bsr, bls, or BSD genes, 50mg (5 x 1ml)
Selective antibiotic for the bsr, bls, or BSD genes, 50mg (5 x 1ml)
STEMCELL Technologies ACCUTASE™, Size: 100 mL
A cell detachment solution of proteolytic and collagenolytic enzymes. Useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware. ACCUTASE™ does not contain mammalian or bacterial-derived products. Each lot of ACCUTASE™ is tested for sterility (by USP membrane filtration method), enzymatic activity (tested with synthetic chromagenic tetrapeptides) and cell detachment from tissue culture plastic.
STEMCELL Technologies Heparin Solution, Size: 2 mL
Heparin is a mucopolysaccharide with anticoagulant properties. It supports the binding of fibroblast growth factor (FGF) to its receptor and increases the stability of FGF. Heparin is used together with epidermal growth factor (EGF) and FGF in NeuroCult™ media for the culture of embryonic rat or adult human neural stem and progenitor cells, and adult mouse neural stem and progenitor cells. For complete instructions, refer to the Technical Manual: In Vitro Proliferation and Differentiation of Neural Stem and Progenitor Cells Using NeuroCult™ (Human; Document #28724 or Mouse/Rat; Document #28725), available at www.stemcell.com or contact us to request a copy. Heparin Solution is also required as a supplement in various other STEMCELL culture media, including MammoCult™ Human Medium Kit (Catalog #05620), EpiCult™-B Mouse Medium Kit (Catalog #05610), and EC-Cult™-XF Culture Kit (Catalog #08000).
Lonza Walkersville MycoAlert PLUS Mycoplasma Detection Kit (30 Tests)
Lonza’s MycoAlert Mycoplasma Detection Assays are selective biochemical tests to detect Mycoplasma contamination in cell cultures. They exploit the activity of mycoplasmal enzymes which are found in the vast majority of about 200 mycoplasma species, but are not present in eukaryotic cells.Benefits:Easy assay: Simply add two reagents to the culture supernatant and perform two luminescence readingsResults in < 20 minEasy interpretation of resultsApplications:Detects all common mollicute contaminations (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma; except Ureaplasma)Suited for tube luminometers, plate luminometers and multifunctional readersContent:3 x 1.2 mL MycoAlert PLUS Reagent (lyophilized)3 x 1.2 mL MycoAlert PLUS Substrate (lyophilized)3 x 3 mL MycoAlert PLUS Assay Buffer
STEMCELL Technologies Hydrocortisone Stock Solution, Size: 3 mL
Hydrocortisone Stock Solution is recommended for use as a cell culture supplement and for the preparation of PneumaCult™, MammoCult™, and EpiCult™ media when culturing airway epithelial cells, mammary primary cells, or tumor cell lines.Hydrocortisone Stock Solution is provided at a concentration of 96 µg/mL, which is ideal as a 200X stock solution for use with PneumaCult™-ALI (Catalog #05001), MammoCult™ (Catalog #05620), EpiCult™-B (Human; Catalog #05601), and EpiCult™-C (Catalog #05630) media. It may also be used for preparing PneumaCult™-Ex Medium (Catalog #05008) or PneumaCult™-Ex Plus Medium (Catalog #05040).
FUJIFILM IRVINE SCIENTIFIC INC BalanCD HEK293 Feed, 500 mL
BalanCD HEK293 Feed is formulated to significantly increase protein yields in suspension-adapted HEK293 cell lines post-transfection.
Invivogen G418 1G LIQUID @ 100MG/ML
G418, 1g Liquid @ 100mg/mL, 5 x 2mL Tubes, Colorless Solution, Shipped at Room Temperature, Stored at -20degreeC, 1 Year, Aminoglycoside Antibiotic, Blocks Polypeptide Synthesis, by Inhibiting Elongation, Both Prokaryotic and Eukaryotic C
STEMCELL Technologies 0.1% Gelatin in Water, Size: 500 mL
This product is recommended for the coating of culture dishes or flasks to grow mouse embryonic stem cells (mESCs), mouse induced pluripotent stem cells (miPSCs) or primary embryonic fibroblasts (PEFs).
STEMCELL Technologies ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator, Size: 2 mL
Achieve robust activation and expansion of T cells in the absence of magnetic beads, feeder cells, or antigens. This product’s gentle activation stimulus ensures a high viability of activated T cells, which can be further expanded in ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981) or other media for culturing human T cells. ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator consists of soluble tetrameric antibody complexes that bind to and cross-link CD3, CD28, and CD2 cell surface ligands, thereby providing the required primary and co-stimulatory signals for T cell activation. This product is designed for cell therapy research applications, but may be qualified for use as an ancillary material (AM) following the framework outlined in USP<1043>. Contact STEMCELL Technologies to learn how we can work with you to qualify this reagent as an AM under an approved investigational new drug (IND) or clinical trial application (CTA).
Sigma Aldrich Fine Chemicals Biosciences EmbryoMax Advanced KSOM Embryo Medium | 50mL
EmbryoMax Advanced KSOM Medium can be used as a single medium to harvest and culture preimplantation mouse embryos, replacing the normal 2-step system where one medium is used for embryo harvest and another is used for culture.
Sigma Aldrich Fine Chemicals Biosciences Liberase TM Research Grade, 10 mg
Liberase TM Research Grade contains highly purified Collagenase I and Collagenase II. These two collagenase isoforms are blended in a precise ratio to each other, and with a medium concentration of Thermolysin, a non-clostridial neutral protease.