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Immunoassays
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BD Biosciences offers a portfolio of immunoassay products for the accurate quantitation and detection of a variety of soluble and intracellular proteins, including cytokines, chemokines, growth factors and phosphorylated cell signaling proteins.

The BD Cytometric Bead Array (CBA) platform is designed to analyze as many as 30 proteins in just 25 to 50µL of sample.  The CBA system uses antibody-coated beads and the fluorescence detection of flow cytometry to efficiently capture analytes. Beads with unique fluorescence intensities can be mixed and used simultaneously in a single tube. BD CBA kits are preconfigured for consistent results for routine panels; BD CBA Flex Sets allow researchers to build customized multiplex panels.


Featured Products

The BD Cytometric Bead Array Enhanced Sensitivity Flex Set system provides a new level of detection compared to other multiplex assay systems. 

Most bead assays can detect cytokine levels of 5 to 10 pg/mL. BD Enhanced Sensitivity Flex Set assays can detect levels as low as 0.274 pg/mL, an improvement of more than 35-fold. The CBA system provides a reliable, flexible method for quantitative detection of multiple proteins in a single serum, plasma or tissue culture supernatant sample. BD CBA Enhanced Sensitivity Flex Sets can detect levels of <1 pg/mL of cytokine.

Flex Sets and Buffer Kits

Catalog No. Description Size

Catalog No.

Description
Human IFN-γ Enhanced Sensitivity Flex Set (Bead B8)
Size
100 tests

Catalog No.

Description
Human IL-1β Enhanced Sensitivity Flex Set (Bead B4)
Size
100 tests

Catalog No.

Description
Human IL-2 Enhanced Sensitivity Flex Set (Bead A4)
Size
100 tests

Catalog No.

Description
Human IL-4 Enhanced Sensitivity Flex Set (Bead A5)
Size
100 tests

Catalog No.

Description
Human IL-5 Enhanced Sensitivity Flex Set (Bead A6)
Size
100 tests

Catalog No.

Description
Human IL-6 Enhanced Sensitivity Flex Set (Bead A7)
Size
100 tests

Catalog No.

Description
Human IL-8 Enhanced Sensitivity Flex Set (Bead A9)
Size
100 tests

Catalog No.

Description
Human IL-10 Enhanced Sensitivity Flex Set (Bead B7)
Size
100 tests

Catalog No.

Description
Human IL-12p70 Enhanced Sensitivity Flex Set (Bead C6)
Size
100 tests

Catalog No.

Description
Human IL-17A Enhanced Sensitivity Flex Set (Bead B5)
Size
100 tests

Catalog No.

Description
Human TNF Enhanced Sensitivity Flex Set (Bead C4)
Size
100 tests

Catalog No.

Description
Human Enhanced Sensitivity Master Buffer Kit
Size
100 tests

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ELISA

Whether relying on pre-optimized, ready-to-use ELISA kits or developing your own sandwich assays, BD provides maximum flexibility. 

BD offers a large menu of optimized BD OptEIA™ kits and sets and allows researchers to optimize ELISA assays by combining individually matched capture and detection antibody pairs with corresponding recombinant protein standards.

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ELISPOT 

BD Biosciences high-quality ELISPOT reagents offer the flexibility to choose among several formats:  Ready-to-use kits with pre-coated BD ELISPOT plates, coat-it-yourself sets and matched antibody pairs. Coupled with a high-throughput ELISPOT plate reader, BD ELISPOT reagents provide objective and rapid analysis of cytokines at the single-cell level.

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T Cell Research
BD Biosciences T-Cell Research Brochure

Multicolor flow cytometry is preeminent among the wide variety of platforms that facilitate T cell studies. With the use of fluorescent antibody panels, this technique allows researchers to characterize cells based on surface or intracellular markers such as cytokines and phosphorylated signaling proteins.

Comprehensive Kits for T Cell Analysis

BD T Cell Analysis Kits provide a simple solution for measuring multiple cytokines or transcription factors from a single sample using multicolor flow cytometry. 

Depending on the kit, researchers can simultaneously examine Th1, Th2 and Th17 cells; just Th1 and Th17 cells; or Th17 and regulatory T cells (in mouse or human model systems). For example, the BD Phosflow™ Human T Cell Activation Kit provides all of the reagents necessary to classify T cells into CD4 and CD8 subsets and to investigate the phosphorylation status of six different signaling proteins.

Other markers can be added to all of the T cell analysis kits to further characterize any subpopulations. The kits also utilize simplified staining protocols, using optimized buffer systems to streamline experimental procedures.

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Cy™ is a trademark of Amersham Biosciences Corp. 

Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

Featured Products

Catalog No. Description Size Components

Catalog No.

Description
BD Pharmingen™ Transcription Factor Buffer Set
Size
100 tests
Components
Fixation/permeabilization buffer, diluent buffer, perm/wash buffer

Catalog No.

Description
Human T Cell Activation Kit
Size
50 tests
Components
Alexa Fluor™ 647 Conjugates: p38 MAPK (pT180/Y182), ERK (pT202/204), Stat1 (pY701), Stat3 (pY705), Stat5 (pY694), and Stat6 (pY641) Human T Cell Cocktail, Compensation Reagents, Control Cells and Buffers

Catalog No.

Description
Human Th1/Th2/Th17 Phenotyping Kit
Size
50 tests
Components
Human Th1/Th2/Th17 Phenotyping Cocktail (CD4 PerCP-Cy*5.5, IFN-γ, FITC, IL-17A PE, IL-4 APC), BD Cytofix™ Fixation Buffer, BD Perm/Wash* Buffer, BD GolgiStop™ Protein Transport Inhibitor

Catalog No.

Description
Human Th1/Th17 Phenotyping Kit
Size
50 tests
Components
Human Th1/Th17 Phenotyping Cocktail (CD4 PerCP-Cy5.5, IFN-γ, FITC, IL-17A PE) BD Cytofix Fixation Buffer, BD Perm/Wash Buffer, BD GolgiStop Protein Transport Inhibitor

Catalog No.

Description
Human Th17/Treg Phenotyping Kit
Size
50 tests
Components
Human Th17/Treg Phenotyping Cocktail (CD4 PerCP-Cy5.5, IL-17A PE, FoxP3 Alexa Fluor 647) FoxP3 Buffer Set, BD GolgiStop Protein Transport Inhibitor

Catalog No.

Description
Mouse Th1/Th2/Th17 Phenotyping Kit
Size
50 tests
Components
Mouse Th1/Th2/Th17 Phenotyping Cocktail (CD4 PerCP-Cy5.5, IFN-γ, FITC, IL-17A PE, IL-4 APC) BD Cytofix Fixation Buffer, BD Perm/Wash Buffer, BD GolgiStop Protein Transport Inhibitor

Catalog No.

Description
Mouse Th1/Th17 Phenotyping Kit
Size
50 tests
Components
Mouse Th1/Th17 Phenotyping Cocktail (CD4 PerCP-Cy5.5, IFN-γ, FITC, IL-17A PE) BD Cytofix Fixation Buffer, BD Perm/Wash Buffer, BD GolgiStop Protein Transport Inhibitor

Catalog No.

Description
Mouse Th17/Treg Phenotyping Kit
Size
50 tests
Components
Mouse Th17/Treg Phenotyping Cocktail (CD4 PerCP-Cy5.5, IL-17A PE, Foxp3 Alexa Fluor 647), Foxp3 Buffer Set, BD GolgiStop Protein Transport Inhibitor
Antibody-Based Techniques

BD Biosciences offers an extensive selection of over 1,400 unconjugated antibodies for use in cellular imaging, microscopy, ELISA, Western blot and an array of functional assays. These tools help researchers investigate immune responses, identify distinct features of cell types and populations and investigate cellular and subcellular interactions in various tissues.

BD Biosciences reagents are known for their quality and reliability and are trusted by researchers around the world. We offer personalized support and consistent tools that accelerate the pace of discovery.

Fluorescent Microscopy

Immunocytochemistry (ICC) and immunohistochemistry (IHC) techniques allow specific markers to be identified in cells and tissues. BD Biosciences cellular imaging reagents have been tested and certified for their utility in automated cellular imaging applications.

Featured Products

Catalog Brand Name Format Clone Apps Buy Now

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BD Pharmingen™
Brand
Caspase-3, Active Form
Name
Purified
Format
C92-605
Clone
Bioimg, ELISA, IC/FCM, IHC(Fr), IP/WB
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BD Pharmingen™
Brand
Ki-67
Name
Purified
Format
B56
Clone
Bioimg, IC/FCM
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Brand
Rb (a.a. 332-344)
Name
Purified
Format
G3-245
Clone
Bioimg, IC/FCM, WB
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BD Pharmingen™
Brand
Ki-67
Name
Alexa Fluor™ 647
Format
B56
Clone
Bioimg
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BD Pharmingen™
Brand
Ki-67
Name
Alexa Fluor™ 488
Format
B56
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Bioimg
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GATA3
Name
Purified
Format
L50-823
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Bioimg, IHC(F)*, WB
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BD Pharmingen™
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H2AX (pS139)
Name
Alexa Fluor™ 647
Format
N1-431
Clone
Bioimg, IC/FCM
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PARP, Cleaved Form
Name
Alexa Fluor™ 647
Format
F21-852
Clone
Bioimg, IC/FCM, IF
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BD Horizon™
Brand
Ig
Name
BV480
Format
Polyclonal
Clone
Bioimg, IF, IHC
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BD Horizon™
Brand
Ig
Name
BV480
Format
Polyclonal
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Bioimg, IF, IHC
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Streptavidin
Name
BV480
Format
Clone
Bioimg, IF, IHC
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Ig
Name
BV421
Format
Polyclonal
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Bioimg, IF, IHC
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Western Blot and Immunoprecipitation

Western blot (WB) and immunoprecipitation (IP) use the specificity of the antibody for its target protein to identify the presence of this protein in a heterogeneous mix of molecules, cell or tissue lysates. BD Biosciences supports these techniques with over 1,500 WB- or IP-validated antibodies.

Featured Products

Catalog No. Brand Name Clone Apps Buy Now

Catalog No.
BD Transduction Laboratories™
Brand
ß-Catenin
Name
14/Beta-Catenin
Clone
IF, IHC, IP, WB
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Brand
E-Cadherin
Name
36/E-Cadherin
Clone
IF, IHC, IP, WB
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E-Cadherin
Name
36/E-Cadherin
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IF, IHC, IP, WB
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Brand
E-Cadherin
Name
36/E-Cadherin
Clone
IF, IHC, IP, WB
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Brand
HIF-1α
Name
54/HIF-1α
Clone
IF, WB
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Pax-5
Name
24/Pax-5
Clone
F, IHC, WB
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Brand
α-Synuclein
Name
42/α-Synuclein
Clone
IIF, WB
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Brand
PMS2
Name
A16-4
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WB
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*IF: Immunofluorescence;  IHC: Immunohistochemistry;  IP: Immunoprecipitation;  WB: Western Blot


Functional Assays

For monoclonal antibodies that impact the functional activity of their antigen (stimulating, blocking or neutralizing), BD Biosciences offers a no-azide, low-endotoxin format (NA/LE) suitable for in vitro and in vivo studies. The majority of our NA/LE products have an endotoxin level of ≤0.01 EU/μg (≤0.001 ng/μg).

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OptiBuild Reagents

Simplified Convenience and Flexibility

BD OptiBuild Reagents

BD OptiBuild™ custom reagents offer more fluorochrome options with the antibodies you need. Whether you want to minimize compensation or add new markers to complex experiments, BD OptiBuild reagents provide flexibility to evaluate new colors and simplify your panel design. Unlike traditional large scale, expensive custom conjugates, these reagents come in convenient 50-μg vials and can be ordered the same way as any catalog reagent. Your products are made on demand, and ship in less than 72 hours.*

*US shipping time is typically overnight. Shipping times vary by region according to shipping schedules. Since production of BD OptiBuild reagents begins immediately after order placement, pending orders cannot be cancelled.

BD OptiBuild Motion Story

Open Up a World of Opportunity with BD OptiBuild

Open Up a World of Opportunity with BD OptiBuild

BD OptiBuild offers nearly 1000 new antibody-dye combinations with thousands more to come.

We know that accessibility is important to driving your scientific goals. BD OptiBuild™ reagents complement the rest of the reagent portfolio, since existing antibody-dye combinations will not be available as BD OptiBuild reagents. Avoid redesigning your panels because the reagents you need are unavailable.

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Apoptosis & Cell Cycle
bd-fluorescent-microscopy

Life, Death and Cell Proliferation 

The balance between cell growth and death (apoptosis) is important for both development and normal tissue homeostasis. Several methods have been developed to assess apoptosis, cell cycle and cell proliferation; and multicolor flow cytometry and fluorescent microscopy have become essential in these studies. BD Biosciences offers a complete portfolio of reagents and tools to support exploration of the cellular features and changes in these processes.

Assessment of Apoptosis 

Changes in the plasma membrane of living cells are key characteristics of the apoptotic process. One indicator of apoptosis is the presence of phosphatidylserine (PS), which is normally located on the cytoplasmic face of plasma membranes. During apoptosis, PS moves to the outer leaflet of the plasma membrane and can be detected using flow cytometry when bound to fluorochrome-labeled Annexin V.

Cell Cycle Analysis

BD Biosciences carries a series of antibodies and kits designed for the detection of proliferating cells by measurement of bromodeoxyuridine (BrdU), an analog of the DNA precursor thymidine used to measure de novo DNA synthesis. During the S phase of the cell cycle (DNA synthesis), BrdU is incorporated into the newly synthesized DNA and can be readily detected by anti-BrdU specific antibodies.

Flow and Detection Kits

Catalog No. Description Size Components

Catalog No.

Description
FITC BrdU Flow Kit
Size
50 tests
Components
FITC-labeled anti-BrdU antibody, 7-AAD, BrdU solution, DNase, BD Cytofix/Cytoperm* Buffer, BD Perm/Wash* Buffer, BD Cytoperm Plus Buffer

Catalog No.

Description
FITC BrdU Flow Kit
Size
200 tests
Components
FITC-labeled anti-BrdU antibody, 7-AAD, BrdU solution, DNase, BD Cytofix/Cytoperm* Buffer, BD Perm/Wash* Buffer, BD Cytoperm Plus Buffer

Catalog No.

Description
APC BrdU Flow Kit
Size
50 tests
Components
APC-labeled anti-BrdU antibody, 7-AAD, BrdU solution, DNase, BD Cytofix/Cytoperm Buffer, BD Perm/Wash Buffer, BD Cytoperm Plus Buffer

Catalog No.

Description
APC BrdU Flow Kit
Size
200 tests
Components
APC-labeled anti-BrdU antibody, 7-AAD, BrdU solution, DNase, BD Cytofix/Cytoperm Buffer, BD Perm/Wash Buffer, BD Cytoperm Plus Buffer

Catalog No.

Description
BrdU In-Situ Detection Kit I
Size
50 tests
Components
Fixation Buffer, Diluent Buffer, BD Retrievagen A Antigen Retrieval Solution, biotinylated anti-BrdU antibody, ready-to-use Streptavidin-Horseradish peroxidase (SAv-HRP) Solution, DAB Buffer, DAB Chromogen, and Control Slides

Catalog No.

Description
BrdU In-Situ Detection Kit II
Size
200 tests
Components
Fixation Buffer, Diluent Buffer, BD Retrievagen A antigen retrieval solution, biotinylated anti-BrdU antibody, ready-to-use Streptavidin-Horseradish peroxidase (SAv-HRP) solution, DAB Buffer, DAB Chromogen, and Control Slides

Catalog No.

Description
Annexin V: FITC Apoptosis Detection Kit I
Size
100 tests
Components
Annexin V-FITC, Propidium Iodide Staining Solution, Annexin V Binding Buffer

Catalog No.

Description
Annexin V: FITC Apoptosis Detection Kit II
Size
100 tests
Components
Purified Recombinant Annexin V, Annexin V-FITC, Propidium Iodide Staining Solution, Annexin V Binding Buffer

Catalog No.

Description
Annexin V: PE Apoptosis Detection Kit
Size
100 tests
Components
Annexin V-PE, 7-AAD, and Annexin V Binding Buffer

Catalog No.

Description
Annexin V: FITC Fluorescence Microscopy Kit
Size
25 slides
Components
Annexin V-FITC, Annexin V Binding Buffer, and PBS Buffer

Catalog No.

Description
BD Pharmingen Apoptosis, DNA Damage and Cell Proliferation Kit
Size
50 tests
Components
BrdU PerCP-Cy5.5, H2AX (pS139) AlexaFluor 647, Cleaved PARP (Asp214) PE; DAPI; BrdU solution; DNase; BD Cytofix/Cytoperm Fixation/ Permeabilization Solution; BD Perm/Wash Buffer

BrdU Antibodies & Solution

Catalog No. Description Size Components

Catalog No.

Description
BrdU Antibody
Size
50 tests
Components
BrdU-PerCP antibody conjugate

Catalog No.

Description
BrdU Antibody
Size
50 tests
Components
BrdU-PerCP-Cy*5.5 antibody conjugate

Catalog No.

Description
BrdU Antibody
Size
50µg
Components
BrdU-V450 antibody conjugate

Catalog No.

Description
BrdU Antibody
Size
100 tests
Components
BrdU-PE antibody conjugate and isotype control

Catalog No.

Description
BrdU Antibody
Size
100 tests
Components
BrdU-FITC antibody conjugate and isotype control

Catalog No.

Description
BrdU Antibody
Size
0.1mg
Components
BrdU purified antibody

Catalog No.

Description
BrdU solution
Size
25mg
Components
10mg/mL BrdU in Dulbecco's phosphate buffered saline

Annexin V

Catalog No. Description Size Components

Catalog No.

Description
Annexin V
Size
25 tests
Components
Annexin V-APC conjugate

Catalog No.

Description
Annexin V
Size
100 tests
Components
Annexin V-APC conjugate

Catalog No.

Description
Annexin V
Size
200 tests
Components
Annexin V-APC conjugate

Catalog No.

Description
Annexin V
Size
100 tests
Components
Annexin V-Biotin conjugate

Catalog No.

Description
Annexin V
Size
200 tests
Components
Annexin V-Biotin conjugate

Catalog No.

Description
Annexin V
Size
100 tests
Components
Annexin V-Cy5 conjugate

Catalog No.

Description
Annexin V
Size
25 tests
Components
Annexin V-FITC conjugate

Catalog No.

Description
Annexin V
Size
100 tests
Components
Annexin V-FITC conjugate

Catalog No.

Description
Annexin V
Size
25 tests
Components
Annexin V-PE conjugate

Catalog No.

Description
Annexin V
Size
100 tests
Components
Annexin V-PE conjugate

Catalog No.

Description
Annexin V
Size
200 tests
Components
Annexin V-PE conjugate

Catalog No.

Description
Annexin V
Size
50 tests
Components
Annexin V-PerCP-Cy5.5 conjugate

Catalog No.

Description
Annexin V
Size
0.1mg
Components
Annexin V-Purified

Catalog No.

Description
Annexin V
Size
50 tests
Components
Annexin V-V450 conjugate

Catalog No.

Description
Annexin V Binding Buffer
Size
50mL
Components
10x Concentrate

Staining Solution

Catalog No. Description Size Components

Catalog No.

Description
7-AAD
Size
2.0mL
Components
Staining Solution

Catalog No.

Description
Propidium Iodide
Size
2.0mL
Components
Staining Solution

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Cy™ is a trademark of Amersham Biosciences Corp.